38132
N-(5-Fluoresceinyl)maleimide
suitable for fluorescence, ≥90% (HPLC), BioReagent
Synonym(s):
5-Maleimido-fluorescein
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About This Item
Empirical Formula (Hill Notation):
C24H13NO7
CAS Number:
Molecular Weight:
427.36
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352111
MDL number:
product line
BioReagent
Quality Level
assay
≥90% (HPLC)
fluorescence
λex 490 nm; λem 518 nm in 0.1 M Tris pH 8.0
suitability
corresponds for coupling to thiols, suitable for fluorescence
storage temp.
2-8°C
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1
InChI
1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H
InChI key
AYDAHOIUHVUJHQ-UHFFFAOYSA-N
General description
N-(5-Fluoresceinyl) maleimide, also known as fluorescein-5-maleimide (FM), is a fluorescent derivatization reagent. The pH during the derivatization reaction plays a critical role.
Application
N-(5-Fluoresceinyl) maleimide or fluorescein-5-maleimide is used as a fluorescent biosensor to detect nanoparticles.
N-(5-Fluoresceinyl) maleimide (5-FM) is used to fluorescence label molecules such as proteins and peptides via their thiol groups.
N-(5-Fluoresceinyl) maleimide (5-FM) is used to fluorescence label molecules such as proteins and peptides via their thiol groups.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
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Vishakha Dastidar et al.
Journal of bacteriology, 189(15), 5550-5558 (2007-05-29)
In gram-negative bacteria, transporters belonging to the resistance-nodulation-cell division (RND) superfamily of proteins are responsible for intrinsic multidrug resistance. Haemophilus influenzae, a gram-negative pathogen causing respiratory diseases in humans and animals, constitutively produces the multidrug efflux transporter AcrB (AcrB(HI)). Similar
Linda Ojemyr et al.
Biochemistry, 48(10), 2173-2179 (2009-01-27)
Proton transport across biological membranes is a key step of the energy conservation machinery in living organisms, and it has been proposed that the membrane itself plays an important role in this process. In the present study we have investigated
Yumiko Takatsuka et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(15), 6559-6565 (2010-03-10)
The AcrB trimeric multidrug efflux transporter of Escherichia coli pumps out a very wide spectrum of compounds. Although minocycline and doxorubicin have been cocrystallized within the large binding pocket in the periplasmic domain of the binding protomer, nothing is known