51536C
RPMI-1640 Medium
with 2.05 mM L-glutamine, with 25mM HEPES, liquid, sterile-filtered, suitable for cell culture
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About This Item
description
for research or for further manufacturing use
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | hybridoma: suitable, cell culture | mammalian: suitable
components
HEPES: 5958 mg/L
L-glutamine: 300 mg/L
NaHCO3: 2000 mg/L
phenol red: 5.310 mg/L
shipped in
ambient
storage temp.
2-8°C
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Application
RPMI-1640 Medium has been used to incubate dissected lung tissue.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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