C1261
Carboxypeptidase A−Agarose
ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas
Synonym(s):
Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
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About This Item
Specific activity:
≥6 units/mL packed gel, 25 °C
Biological source:
enzyme from bovine pancreas
biological source
enzyme from bovine pancreas
Quality Level
form
ammonium sulfate suspension
specific activity
≥6 units/mL packed gel, 25 °C
mol wt
~35,250
matrix
beaded agarose
storage temp.
2-8°C
General description
Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.
Biochem/physiol Actions
Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.
Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.
Physical form
Suspension in 2.0 M (NH4)2SO4, pH 7
Other Notes
One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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