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Merck

C5405

Chicken Serum

USA origin, sterile-filtered, suitable for cell culture

Synonym(s):

chicken sera

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About This Item

NACRES:
NA.71
UNSPSC Code:
12352207
Biological source:
chicken
Origin:
USA origin
Sterility:
sterile-filtered
Form:
liquid
Shipped in:
dry ice
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biological source

chicken

Quality Level

sterility

sterile-filtered

form

liquid

composition

hemoglobin, ≤60 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

Application

Chicken Serum has been used:
  • for pellet resuspension following centrifugation, to prepare zymosan suspension for respiratory burst assay
  • as a supplement to Dulbecco′s modified Eagle′s medium (DMEM) to culture HeLa P cells
  • for blocking in immunofluorescence (IF) double-staining

Chicken serum is used in parasitology to study excystation, growth, survival and egg production of the microphallid trematode Maritrema novaezealandensis. Chicken serum is used for the serial cultivation of chicken keratinocytes.

Biochem/physiol Actions

Animal sera serve as a cost-effective alternative to basal cell culture media supplement. They are commonly used in immunodetection protocols such as immunohistochemistry (IHC), immunocytochemistry (ICC), and western blot to block nonspecific binding. Chicken serum is suitable for use in parasitology to study excystation, growth, survival, and egg production of the microphallid trematode Maritrema novaezealandensis. It is used for the serial cultivation of chicken keratinocytes.

Analysis Note

Endotoxin tested


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Géraldine Rouquet et al.
Journal of bacteriology, 191(13), 4427-4440 (2009-04-21)
We identified a carbohydrate metabolic operon (frz) that is highly associated with extraintestinal pathogenic Escherichia coli (ExPEC) strains. The frz operon codes for three subunits of a phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) transporter of the fructose subfamily, for a transcriptional activator
Amandine Vanhoutteghem et al.
Differentiation; research in biological diversity, 72(4), 123-137 (2004-05-26)
The epidermis of birds differs from that of mammals by the presence of intracellular lipid droplets and the absence of sebaceous glands. We describe here the cultivation of chicken epidermal keratinocytes; these cells cannot be grown in medium supplemented with
B L Fredensborg et al.
Parasitology research, 95(5), 310-313 (2005-02-01)
In this study, the effect of the composition of culture medium on excystation, growth, survival and egg production was investigated for the recently discovered microphallid trematode Maritrema novaezealandensis. Metacercariae from the two second intermediate crab hosts, Macrophthalmus hirtipes and Halicarcinus