D2906
DMEM/F-12
With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
Synonym(s):
DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
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About This Item
Product Name
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM
shipped in
ambient
storage temp.
2-8°C
General description
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:
- Hep-2 cells
- mouse fibroblast L‐cells
- L‐ Wnt family member 3A (Wnt3a) cells
- primary buffalo embryonic fibroblast (BEF)
Preparation Note
Formulated to contain 15.6 grams of powder per liter of medium.
Supplement with 1.2 g/L sodium bicarbonate.
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Storage Class
11 - Combustible Solids
wgk
WGK 1
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C Zhang et al.
Osteoarthritis and cartilage, 26(6), 824-833 (2018-03-20)
Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of
Nereo Kalebic et al.
EMBO reports, 17(3), 338-348 (2016-01-14)
We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into
Eric M Erkenbrack et al.
PLoS biology, 16(8), e2005594-e2005594 (2018-08-25)
Among animal species, cell types vary greatly in terms of number and kind. The number of cell types found within an organism differs considerably between species, and cell type diversity is a significant contributor to differences in organismal structure and