HT501850
Formalin solution, neutral buffered, 10%
histological tissue fixative
Synonym(s):
10% NBF, 10% Neutral Buffered Formalin, Formaldehyde, Methanal
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About This Item
biological source
synthetic
Quality Level
concentration
10% formalin (approx. 4% formaldehyde)
color
colorless
pH
6.90-7.10
density
1.080 g/cm3
application(s)
hematology
histology
storage temp.
room temp
SMILES string
[O-]#[C+H2]
InChI
1S/CH2O/c1-2/h1H2
InChI key
WSFSSNUMVMOOMR-UHFFFAOYSA-N
General description
10% Neutral Buffered Formalin is the most used fixative throughout the world for light microscopy. Formalin solution is the commercially obtained solution containing 37% w/w (40% w/v) of formaldehyde in water. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.
Application
Popular general purpose histological fixative.
Formalin solution (neutral buffered, 10%) has been popularly used as a general-purpose histological fixative to fix tissue samples.
- It effectively preserves the cellular and tissue morphology, maintaining the structural integrity required for microscopic examination.
- It may be used as a reagent for the primary fixation of tissues for diagnostic applications of an immunoperoxidase method in hematopathology.
- It is used for routine histological staining, providing clear and detailed images of cellular structures.
Biochem/physiol Actions
Formaldehyde is both a non-coagulant and an additive fixative. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins.
Features and Benefits
- 10% NBF is well-suited for large throughput laboratories.
- It requires a relatively short period of fixation.
- It can be used for the long-term storage of tissue.
Physical form
Neutral buffered solution
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Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Muta. 2 - Skin Sens. 1
wgk
WGK 3
ppe
Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
185.0 °F
flash_point_c
85 °C
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Diagnostic applications of an immunoperoxidase method in hematopathology.
Banks PM.
The Journal of Histochemistry and Cytochemistry, 27(8), 1192-1194 (1979)
Histological and Histochemical Methods ? Theory and practice, 5th Edition
J. A. Kiernan
Histological and Histochemical Methods: Theory and Practice (2015)
Jaehwan Ahn et al.
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