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M3671

MES

Name: MES
Brand: SIGMA

low moisture content, ≥99% (titration)

Synonym(s):

2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):

C₆H₁₃NO₄S

CAS Number:

4432-31-9

Molecular Weight:

195.24

NACRES:

NA.25

PubChem Substance ID:

24896867

eCl@ss:

32129211

UNSPSC Code:

12161700

EC Number:

224-632-3

MDL number:

MFCD00006181

Beilstein/REAXYS Number:

141319

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Properties

Quality Level

400

assay

≥99% (titration)

form

powder

storage condition

dry at room temperature

impurities

≤1.0% water

color

white

pH

2.5-4 (25 °C, 97.6 g/L)

useful pH range

5.5-6.7

pKa

6.1

density

1.23 g/cm³ at 20—25 °C, 1.27 g/cm³ at 20—25 °C

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma (
)
metabolomics (
)

SMILES string

OS(=O)(=O)CCN1CCOCC1

InChI

1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)

InChI key

SXGZJKUKBWWHRA-UHFFFAOYSA-N

Description

General description

MES Buffer, recognized as one of the "Good" buffers employed in biological and biochemical applications, plays a crucial role across various research domains. This zwitterionic and morpholinic buffer proves highly valuable due to its effective buffering capacity within the pH range of 5.5 to 6.7. Notably, MES Buffer does not engage in complex formation with the majority of metals commonly employed in environmental and biological studies. Its solubility in water is excellent, and it exhibits minimal lipid solubility, rendering it impermeable to membranes.

This versatile buffer may find widespread application in maintaining a stable environment in solutions dedicated to cell culture media, protein-based buffer formulations, and electrophoresis running buffers. Its significance extends to the purification processes of antibodies, peptides, proteins, blood components, and growth factors. MES Buffer stands out as an optimal choice for capillary electrochromatography, offering low ionic mobility. Notably, it serves as a safer alternative to potentially toxic agents like cacodylate and non-zwitterionic buffers, including citrate and malate. MES Buffer also proves valuable for fine-tuning the pH of growth media, stabilizing enzymatic solutions, and serving as a component of PAGE running buffer in diverse experimental settings.

Application

It has been utilized as a buffer bicarbonate‐containing culture medium

MES has been used as a component of the elution buffer used to elute plasma from α2-macroglobulin affinity column. It has been used as an activation buffer during antibody conjugation to microparticles. MES has been used in the functionalization of microchannels during the creation of microfluidic-integrated surface plasmon resonance (SPR) platform for pathogen detection.

Features and Benefits

Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Effective Buffering from pH 5.5-6.7 (25 °C) with a pKa of 6.1 (25 °C)
Highly soluble in water
Minimal metal ion binding
Less toxic to cells than other buffers such as Tris and phosphate
Stable in a wide pH range
Low UV absorptivity and Minimal reactivity

Analysis Note

Low moisture

Other Notes

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Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

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