M7653
Medium 199
With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Medium 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: yes
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
phenol red: yes
HEPES: no
shipped in
ambient
storage temp.
2-8°C
General description
Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Modification of the original Medium 199.Earle′s salts are replaced with Hanks′ salts. Hanks′ salts use a lower concentration of bicarbonate and different concentrations and salt forms of the essential inorganic ions.
Preparation Note
Supplement with 0.1 g/L L-glutamine.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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