N8271
α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
Neuraminidase from Arthrobacter ureafaciens, Acyl-neuraminyl Hydrolase, Sialidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
Specific activity:
≥135 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
form
buffered aqueous solution
specific activity
≥135 units/mg protein
mol wt
88 kDa, 95 kDa
foreign activity
β-Galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, and proteases, none detected
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Releases α(2→3), α(2→6), α(2→8), and α(2→9)-linked N-acetylneuraminic acid from complex oligosaccharides.
Packaging
Provided with 5× reaction buffer (250 mM sodium phosphate, pH 6.0).
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.
Preparation Note
Expressed in glycosidase-free hosts.
Other Notes
One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37 °C.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Instructions
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