T9028
Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse
clone TUB-1A2, ascites fluid
Synonym(s):
Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5
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About This Item
Conjugate:
unconjugated
Clone:
TUB-1A2, monoclonal
Application:
ARR, IF, WB
Species reactivity:
human, plant, animal
Citations:
171
Technique(s):
indirect immunofluorescence: 1:800 using cultured chicken fibroblasts, microarray: suitable, western blot: suitable
Uniprot accession no.:
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
TUB-1A2, monoclonal
contains
15 mM sodium azide
species reactivity
human, plant, animal
technique(s)
indirect immunofluorescence: 1:800 using cultured chicken fibroblasts, microarray: suitable, western blot: suitable
isotype
IgG3
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBA4A(7277), TUBB(203068)
Application
Monoclonal Anti-Tyrosine Tubulin has been used in:
- indirect immunofluorescent labelling
- immunoblotting technique
- immunocytochemical staining
Biochem/physiol Actions
The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.
Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology.
The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus.
Immunogen
peptide containing the carboxy-terminal amino acids of α-tubulin
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
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Nagayasu Nakanishi et al.
BMC biology, 12, 26-26 (2014-04-01)
The evolutionary origin of gastrulation--defined as a morphogenetic event that leads to the establishment of germ layers--remains a vexing question. Central to this debate is the evolutionary relationship between the cell layers of sponges (poriferans) and eumetazoan germ layers. Despite
Microtubule Structures
Molecular Cell Biology. 4th edition. (2000)
L J Lee et al.
Journal of cellular biochemistry, 81(1), 162-171 (2001-02-17)
Conflicting data for the effects of colchicine on cholesterol transport and steroidogenesis raise the question of the role of microtubules in cholesterol transport from the lipid droplet to mitochondria in steroidogenic cells. In this study, using corticosterone radioimmunoassay and immunofluorescence
Malkolm Graffe et al.
The Journal of general physiology, 146(1), 109-117 (2015-07-01)
A set of bipolar cells in the retina of goldfish contains giant synaptic terminals that can be over 10 µm in diameter. Hundreds of thousands of synaptic vesicles fill these terminals and engage in continuous rounds of exocytosis. How the
Yasuharu Takaku et al.
Scientific reports, 4, 3573-3573 (2014-01-08)
Nerve cells and spontaneous coordinated behavior first appeared near the base of animal evolution in the common ancestor of cnidarians and bilaterians. Experiments on the cnidarian Hydra have demonstrated that nerve cells are essential for this behavior, although nerve cells
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