05-623
Anti-RNA polymerase II Antibody, clone CTD4H8
clone CTD4H8, Upstate®, from mouse
Synonym(s):
Anti-NEDHIB, Anti-POLR2, Anti-POLRA, Anti-RPB1, Anti-RPBh1, Anti-RPO2, Anti-RPOL2, Anti-RpIILS, Anti-hRPB220, Anti-hsRPB1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-RNA polymerase II Antibody, clone CTD4H8, clone CTD4H8, Upstate®, from mouse
Quality Level
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CTD4H8, monoclonal
species reactivity
Saccharomyces cerevisiae, human, mouse, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... POLR2A(5430)
mouse ... Polr2A(20020)
rat ... Polr2A(363633)
Application
Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Biochem/physiol Actions
This antibody recognizes Phospho- and non-phospho-RNA polymerase II.
Analysis Note
Control
A431 nuclear extract.
A431 nuclear extract.
Routinely evaluated by western blot on HeLa nuclear extracts.
Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
210-220 kDa
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Immunogen
Peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Chromatography
Purified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the {beta}-globin gene locus.
Zhou Z, Li X, Deng C, Ney PA, Huang S, Bungert J
The Journal of Biological Chemistry null
Sp1 and Sp3 foci distribution throughout mitosis.
He, Shihua and Davie, James R
Journal of Cell Science, 119, 1063-1070 (2006)
Coordination of replication and transcription along a Drosophila chromosome.
MacAlpine, DM; Rodriguez, HK; Bell, SP
Genes & Development null
M Patturajan et al.
The Journal of biological chemistry, 274(39), 27823-27828 (1999-09-17)
Monoclonal antibodies that recognize specific carboxyl-terminal domain (CTD) phosphoepitopes were used to examine CTD phosphorylation in yeast cells lacking carboxyl-terminal domain kinase I (CTDK-I). We show that deletion of the kinase subunit CTK1 results in an increase in phosphorylation of
Correlation of dysfunction of nonmuscle myosin IIA with increased induction of Cyp1a1 in Hepa-1 cells.
Ebina M, Shibazaki M, Kudo K, Kasai S, Kikuchi H
Biochimica et Biophysica Acta null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-623 | 04053252511127 |
| 05-623-25UG | 04054839350191 |
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