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1.05454

Brilliant-Green Bile Lactose Broth

GranuCult® prime, according to ISO 4831, ISO 4832, APHA, FDA-BAM, granular, for Escherichia coli, for coliforms, pkg of 500 g, pkg of 5 kg

Synonym(s):

BGB broth, BGBL broth, BGBLB, BRILA (Brilliant-green bile Lactose) broth, Brilliant Green Bile broth, Brilliant-green bile lactose broth, Brillant-green bile lactose broth

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
GranuCult® prime
Agency:
APHA, FDA-BAM, ISO 4832, according to ISO 4831
Ph:
7.2 (30 °C, 40 g/L in H2O, after autoclaving)
Form:
granular
Suitability:
Escherichia coli, coliforms
Application(s):
food and beverages
microbiology
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Quality Level

200

agency

APHA, FDA-BAM, ISO 4832, according to ISO 4831

sterility

non-sterile

form

granular

packaging

pkg of 5 kg, pkg of 500 g

manufacturer/tradename

GranuCult® prime

storage condition

dry media (Keep tightly closed)

technique(s)

microbiological culture: suitable

color

green

pH

7.2 (30 °C, 40 g/L in H2O, after autoclaving)

solubility

40 g/L

bulk density

560 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Escherichia coli, coliforms

General description

GranuCult® prime Brilliant-green bile Lactose broth contains brilliant green and bile as the inhibitory agents for Gram-positive organisms. Lactose is a carbon source, which is dissimilated rapidly by the coli-aerogenes group, mostly by the heterofermentative pathway, leading to gas formation. It is a widespread practice to perform preliminary MPN tests using a less selective medium such as Lauryl Sulfate broth (1.10266) acc. ISO 4831 and FDA‑BAM and confirm any tube showing a positive reaction by subculture to BRILA broth. The medium is also used for confirmation after pour plating with Violet Red Bile Lactose agar acc. ISO 4832 and FDA‑BAM.

Application

GranuCult® prime BRILA (Brilliant-green bile Lactose) broth is suitable for the selective enrichment, enumeration, and confirmation of Escherichia coli and other fecal coliform organisms from food and animal feed, water, and other materials.

Features and Benefits

  • GranuCult® offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

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hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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