Anti-Orexin Antibody, Prepro

A 17 mer peptide (designated PPOXA11-A) mapping near the C-terminus of mouse prepro-Orexin.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 1:10,000 - 100,000

Immunohistochemistry: 2-20 μg/mL.

Optimal working dilutions must be determined by end user.

Anti-Orexin Antibody, Prepro is an antibody against Orexin for use in ELISA, IH & WB.

Mouse Prepro-Orexin. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Liquid in PBS with 0.1% BSA.

Can be refrigerated at 2-8°C in undiluted aliquots for short term use. Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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