MAB4381
Anti-TRA-1-81 Antibody, clone TRA-1-81
clone TRA-1-81, Chemicon®, from mouse
Synonym(s):
Clone TRA-1-81 Antibody, TRA-1-81 Detection Antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
TRA-1-81, monoclonal
Species reactivity:
human
Application:
FACS, ICC, IF, IP, WB
Citations:
276
biological source
mouse
Quality Level
antibody form
saturated ammonium sulfate (SAS) precipitated
antibody product type
primary antibodies
clone
TRA-1-81, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
input
sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
General description
420/250 kDa
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.
Immunogen
Human embryonal carcinoma cell line 2102Ep
Application
Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.
Immunofluorescence: A previous lot of this antibody was used in IF.
Western Blot: A previous lot of this antibody was used in WB.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: A previous lot of this antibody was used in IH.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Western Blot: A previous lot of this antibody was used in WB.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: A previous lot of this antibody was used in IH.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
Biochem/physiol Actions
This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
Physical form
Format: Purified
Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.
Preparation Note
Stable for 1 year at from date of receipt.
Analysis Note
Control
Human embryonic stem cells
Human embryonic stem cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
C Eguizabal et al.
Stem cells (Dayton, Ohio), 29(8), 1186-1195 (2011-06-18)
Gamete failure-derived infertility affects millions of people worldwide; for many patients, gamete donation by unrelated donors is the only available treatment. Embryonic stem cells (ESCs) can differentiate in vitro into germ-like cells, but they are genetically unrelated to the patient.
Expression of pluripotent stem cell markers in the human fetal ovary.
Kerr, CL; Hill, CM; Blumenthal, PD; Gearhart, JD
Human Reproduction null
Tomoko Andoh-Noda et al.
Molecular brain, 8, 31-31 (2015-05-28)
Rett syndrome (RTT) is one of the most prevalent neurodevelopmental disorders in females, caused by de novo mutations in the X-linked methyl CpG-binding protein 2 gene, MECP2. Although abnormal regulation of neuronal genes due to mutant MeCP2 is thought to
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB4381 | 04053252616716 |