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Merck

21878

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester

BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

Synonym(s):

5(6)-FAM SE, FLUOS

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About This Item

Empirical Formula (Hill Notation):
C25H15NO9
CAS Number:
117548-22-8
Molecular Weight:
473.39
NACRES:
NA.32
PubChem Substance ID:
57647947
UNSPSC Code:
12352108
MDL number:
MFCD04119713

Key Documents

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Product Name

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester, BioReagent, suitable for fluorescence, mixture of isomers, ≥80% (HPLC)

InChI

1S/2C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)18-9-12(1-4-15(18)24(31)32)25(33)35-26-21(29)7-8-22(26)30;27-12-4-6-14-18(10-12)34-19-11-13(28)5-7-15(19)22(14)23-16(24(31)32)2-1-3-17(23)25(33)35-26-20(29)8-9-21(26)30/h1-6,9-11,27H,7-8H2,(H,31,32);1-7,10-11,27H,8-9H2,(H,31,32)

SMILES string

OC(=O)c1ccc(cc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O.OC(=O)c6cccc(C(=O)ON7C(=O)CCC7=O)c6C8=C9C=CC(=O)C=C9Oc%10cc(O)ccc8%10

InChI key

YVTPVJDECOUTJW-UHFFFAOYSA-N

product line

BioReagent

assay

≥80% (HPLC)

form

powder

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in DMF

suitability

suitable for fluorescence

storage temp.

−20°C

Quality Level

200

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Application

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5(6)-FAM SE, FLUOS) may be used to prepare fluorescent derivatives of proteins such as tubulin and for the preparation of a phenytoin tracer for the determination of this and other drugs in serum and plasma by fluorescence polarization immunoassay (FPIA) and laser-induced fluorescence detection.
Fluorescent label for primary and secondary amine groups on proteins and other biomolecules.
Has been used to prepare fluorescent derivatives of tubulin and for the preparation of a phenytoin tracer for the determination of this drug in serum and plasma by fluorescence polarization immunoassay (FPIA)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCJ4486
BCCG1301
BCCD9960
BCBZ6041
BCBV1325

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