Skip to Content
Merck

B6916

Bradford Reagent

for 0.1-1.4 mg/ml protein

Synonym(s):

Coomassie dye binding protein assay, Coomassie dye binding protein assay, Protein dye reagent, Protein dye reagent

Sign In to View Organizational & Contract Pricing.

Select a Size


About This Item

NACRES:
NA.32
UNSPSC Code:
12161500
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

solution

storage temp.

2-8°C

Quality Level

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.

Application

Bradford Reagent has been used to determine total protein concentration.

Features and Benefits

  • The reagent is ready to use. No mixing or dilution required.
  • Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
  • Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
  • Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
  • Can be used in microwell plate assays.
  • Inexpensive assay.

Legal Information

pictograms

Health hazardCorrosion

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

target_organs

Eyes,Central nervous system

Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.
Charoenwong D et al.
Applied and Environmental Microbiology, 77, 5220-5229 (2011)
Proteomic response of the biological control fungus Trichoderma atroviride to growth on the cell walls of Rhizoctonia solani.
Grinyer J et al.
Current Genetics, 47, 381-381 (2005)
Rosenberg IM
Protein Analysis and Purification: Benchtop Techniques (2006)
Tracey Welham et al.
Journal of experimental botany, 60(12), 3353-3365 (2009-05-29)
Neutral/alkaline invertases are a subgroup, confined to plants and cyanobacteria, of a diverse family of enzymes. A family of seven closely-related genes, LjINV1-LjINV7, is described here and their expression in the model legume, Lotus japonicus, is examined. LjINV1 previously identified
Sin-Jin Li et al.
Clinical nutrition (Edinburgh, Scotland), 36(3), 760-767 (2016-06-28)
The cellular mechanisms of obesity-induced cardiomyopathy are multiple and not completely elucidated. The objective of this study was to differentiate two obesity-associated cardiomyopathy miniature pig models: one with the metabolic syndrome (MetS), and one with a metabolically healthy obesity (MHO).

Related Content

We have developed a novel, high-binding capacity, antibodybased resin for the depletion of twenty high abundance proteins in human plasma. The technology is a significant improvement over existing kits that remove 2, 6, or 12 proteins.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service