P8913
Phospholipase A2 from bovine pancreas
lyophilized powder, ≥20 units/mg protein
Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-637-7
MDL number:
Specific activity:
≥20 units/mg protein
Product Name
Phospholipase A2 from bovine pancreas, lyophilized powder, ≥20 units/mg protein
form
lyophilized powder
specific activity
≥20 units/mg protein
composition
Protein, 5-20% biuret
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
cow ... PLA2G1B(282457)
Looking for similar products? Visit Product Comparison Guide
Analysis Note
Protein determined by biuret.
Application
Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.
Biochem/physiol Actions
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.
General description
Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.
Other Notes
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.
Packaging
Package size based on protein content
Physical form
Contains Tris buffer salts.
wgk
WGK 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Renal tubular triglyercide accumulation following endotoxic, toxic, and ischemic injury.
Zager RA
Kidney International, 67(1), 111-121 (2005)
Triglyceride accumulation in injured renal tubular cells: alterations in both synthetic and catabolic pathways.
Johnson AC
Kidney International, 67(6), 2196-2209 (2005)
Phospholipase A2 activity can protect renal tubules from oxygen deprivation injury.
Zager RA
Proceedings of the National Academy of Sciences of the USA, 90(17), 8297-8301 (1993)
P K Larsson et al.
The Journal of biological chemistry, 273(1), 207-214 (1998-02-07)
Recently, the cloning of a novel Ca2+-independent phospholipase A2 (iPLA2) from Chinese hamster ovary cells as well as from mouse and rat sources containing a C-terminal lipase motif and eight N-terminal ankyrin repeats has been described. In this report we
H M Verheij et al.
Biochimica et biophysica acta, 747(1-2), 93-99 (1983-09-14)
The complete amino acid sequence of phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) from human pancreas was determined. The protein consists of a single polypeptide chain of 125 amino acids and has a molecular weight of 14003. The chain is cross-linked
Related Content
Product Information Sheet
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service