T2885
3,3′,5,5′-Tetramethylbenzidine
TMB substrate, chromogenic, ≥98% (TLC), powder
Synonym(s):
BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Linear Formula:
[-C6H2(CH3)2-4-NH2]2
CAS Number:
Molecular Weight:
240.34
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
259-364-6
MDL number:
Beilstein/REAXYS Number:
2808541
Product Name
3,3′,5,5′-Tetramethylbenzidine, ≥98% (TLC)
Quality Level
assay
≥98% (TLC)
form
powder
mp
168-171 °C (lit.)
solubility
ethyl acetate: 50 mg/mL, clear to very slightly hazy
storage temp.
2-8°C
SMILES string
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key
UAIUNKRWKOVEES-UHFFFAOYSA-N
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
3,3′,5,5′-Tetramethylbenzidine has been used:
- as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2
- as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
- in myeloperoxidase activity assay to quantify neutrophil
Biochem/physiol Actions
3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Still not finding the right product?
Explore all of our products under 3,3′,5,5′-Tetramethylbenzidine
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Instructions
Ferrotransferrin and antibody against the transferrin receptor as potential vehicles for drug delivery across the mammalian blood-brain barrier into the central nervous system
Methods in Neurosciences, 21(1), 93-117 (1994)
Lactobacillus casei improves resistance to pneumococcal respiratory infection in malnourished mice
Villena J, et al.
The Journal of Nutrition, 135(6), 1462-1469 (2005)
Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments
Yamaoka H, et al.
Letters in Applied Microbiology, 62(1), 47-54 (2016)