U8501

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Name: Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast
Brand: SIGMA

Type X, lyophilized powder, ≥40 units/mg protein

Synonym(s):

UTP:α-D-glucose-1-phosphate uridylyltransferase

Select a Size

All Photos(1)

About This Item

CAS Number:

9026-22-6

EC Number:

2.7.7.9 (BRENDA, IUBMB)

UNSPSC Code:

12352204

EC Number:

232-811-2

NACRES:

NA.54

MDL number:

MFCD00132561

Product Name

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast, Type X, lyophilized powder, ≥40 units/mg protein

biological source

bakers yeast

type

Type X

form

lyophilized powder

specific activity

≥40 units/mg protein

composition

Protein, 30-60% modified Warburg-Christian

foreign activity

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Application

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:

in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-¹³C9
to quantify Suc synthase (SUS) enzyme activity in rice
to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes

Biochem/physiol Actions

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg²⁺, Ca²⁺, Mn²⁺, Ni²⁺ for its activity.

General description

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.

Other Notes

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized, sulfate-free powder containing citrate buffer salt

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Dissemination of an erythromycin-resistant penicillin-nonsusceptible Streptococcus pneumoniae Poland(6B)-20 clone in Argentina.

Laura Bonofiglio et al.

Microbial drug resistance (Larchmont, N.Y.), 17(1), 75-81 (2010-12-07)

Prevalence of serotype 6B penicillin (PEN)-nonsusceptible Streptococcus pneumoniae significantly increased from 15.8% (1993-1997) to 67.3% (1998-2002) (p<0.001) in Argentina. Serogroup 6 ranks fourth among different capsular types within invasive isolates from Argentinean patients <6 years of age. To evaluate whether

Building a kinetic model of trehalose biosynthesis in Saccharomyces cerevisiae.

Kieran Smallbone et al.

Methods in enzymology, 500, 355-370 (2011-09-29)

In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast.

The estimation of pyrophosphate in urine with uridine-5'-diphosphoglucose pyrophosphorylase.

D J Sutor et al.

Clinica chimica acta; international journal of clinical chemistry, 86(3), 329-332 (1978-06-15)

The concentration of pyrophosphate in urine has been determined using the enzyme uridine-5'-diphosphoglucose pyrophosphorylase. The technique is relatively quick and easy to perform. Reproducibility of results was good, and results from recovery experiments were excellent. The mean concentration of pyrophosphate

Molecular cloning and analysis of the UDP-Glucose Pyrophosphorylase in Streptococcus equi subsp. zooepidemicus.

Zhe Ma et al.

Molecular biology reports, 38(4), 2751-2760 (2010-11-26)

UDP-Glucose Pyrophosphorylase (EC 2.7.7.9, UGPase) plays an important role in Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) cell envelope Hyaluronic acid (HA) biosynthesis and it is also recognized as a virulence determinant in several bacterial species. HA is valuable biopolymer used

A galU mutant of Francisella tularensis is attenuated for virulence in a murine pulmonary model of tularemia.

Himangi R Jayakar et al.

BMC microbiology, 11, 179-179 (2011-08-09)

A number of studies have revealed that Francisella tularensis (FT) suppresses innate immune responses such as chemokine/cytokine production and neutrophil recruitment in the lungs following pulmonary infection via an unidentified mechanism. The ability of FT to evade early innate immune

View All Related Papers