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Merck

83689

Rhodamine B

suitable for fluorescence

Synonym(s):

Basic Violet 10, Brilliant Pink B, Rhodamine O, Tetraethylrhodamine

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About This Item

Empirical Formula (Hill Notation):
C28H31ClN2O3
CAS Number:
81-88-9
Molecular Weight:
479.01
EC Number:
201-383-9
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
57652962
MDL number:
MFCD00011931
Colour Index Number:
45170
Beilstein/REAXYS Number:
4091619

Product Name

Rhodamine B, suitable for fluorescence

InChI key

PYWVYCXTNDRMGF-UHFFFAOYSA-N

InChI

1S/C28H30N2O3.ClH/c1-5-29(6-2)19-13-15-23-25(17-19)33-26-18-20(30(7-3)8-4)14-16-24(26)27(23)21-11-9-10-12-22(21)28(31)32;/h9-18H,5-8H2,1-4H3;1H

SMILES string

[Cl-].CCN(CC)c1ccc2c(OC3=CC(\C=CC3=C2c4ccccc4C(O)=O)=[N+](/CC)CC)c1

form

powder or crystals

Quality Level

200

mol wt

479.01 g/mol

concentration

80-100 % (w/w) (Rhodamine B)

color

green to very dark green

mp

210-211 (dec.) (lit.)

solubility

H2O: soluble
methanol: soluble

density

0.79 g/cm3

fluorescence

λex 553 nm; λem 627 nm in methanol

suitability

in accordance for fluorescence
suitable for fluorescence

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Analysis Note

λmax. ∼543 nm, E(1%/1cm) ∼2200-2800 (Methanol)

Application

Rhodamine B has been used:
  • as a fluorescent dye for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)
  • as a fluorescent dye in confocal microscopy
  • to study its fluorescence in water and ethanol solutions

for cross staining of N-methylamino-5-isocyanonaphthalene (MICAN)

General description

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a staining fluorescent dye.
Rhodamine B is bright-red in color.

pictograms

CorrosionExclamation mark
GHS05,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM0999
BCCK7198
BCCJ6891
BCCF9755
BCCF1067

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The
A study of water circulation in Monterey harbor using rhodamine B dye (1965)
Simplifying microfluidic separation devices towards field-detection of blood parasites
Holm SH, et al.
Analytical Methods : Advancing Methods and Applications, 8(16), 3291-3300 (2016)
MICAN, a new fluorophore for vital and non-vital staining of human cells
Nagy Z, et al.
Toxicology in vitro, 48, 137-145 (2018)
Rhodamine B as a probe for phase transition in liquid solutions
Shishkin I, et al.
arXiv (2016)
The fluorescence properties of cationic rhodamine B in the gas phase
Sagoo SK and Jockusch RA
Journal of Photochemistry and Photobiology A: Chemistry, 220(2-3), 173-178 (2011)
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