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B2643

Biotinamidohexanoic acid N-hydroxysuccinimide ester

Name: Biotinamidohexanoic acid N-hydroxysuccinimide ester
Brand: SIGMA

≥98% (TLC), powder

Synonym(s):

(+)-Biotinamidocaproate N-hydroxysuccinimidyl ester, N-(+)-Biotinyl-6-aminocaproic acid N-succinimidyl ester, N-(+)-Biotinyl-6-aminohexanoic acid NHS ester, N-Succinimidyl N-biotinyl-6-aminocaproate, Succinimidyl 6-(biotinamido)hexanoate

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₃₀N₄O₆S

CAS Number:

72040-63-2

Molecular Weight:

454.54

UNSPSC Code:

12352203

NACRES:

NA.46

PubChem Substance ID:

24891665

MDL number:

MFCD00065502

Beilstein/REAXYS Number:

3577403

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Properties

Quality Level

200

assay

≥98% (TLC)

form

powder

solubility

H₂O: ≤2 mg/mL (with sonication), DMF: 50 mg/mL (Stable up to 2 months in dry DMF.)

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2

InChI

1S/C20H30N4O6S/c25-15(7-4-3-6-14-19-13(12-31-14)22-20(29)23-19)21-11-5-1-2-8-18(28)30-24-16(26)9-10-17(24)27/h13-14,19H,1-12H2,(H,21,25)(H2,22,23,29)/t13-,14-,19-/m0/s1

InChI key

UVGHPGOONBRLCX-NJSLBKSFSA-N

Description

Application

Biotinamidohexanoic acid N-hydroxysuccinimide ester has been used in the biotinylation of antibodies.

Biochem/physiol Actions

Biotin is a label widely used in several biochemical applications and has a high affinity for avidin. A spacer chain is required to reduce steric hindrance while coupling biotin (a small molecule) to biopolymers (large molecules). Biotinamidohexanoic acid N-hydroxysuccinimide ester incorporates a 7-atom spacer with the N-succinimide leaving group. It can react with a primary amine in the pH range of 6.5-8.5. This biotinylation reagent incorporates an aminocaproyl spacer and thus can reduce the steric hindrance in binding avidin to some biotinylated compounds.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000542313

0000514169

0000496534

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