H0163
Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal
clone HDAC4-144, purified from hybridoma cell culture
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
Conjugate:
unconjugated
Clone:
HDAC4-144, monoclonal
Application:
ICC, IP, WB
Citations:
3
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HDAC4-144, monoclonal
form
buffered aqueous solution
mol wt
antigen ~140 kDa
species reactivity
human, rat, mouse
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~2 mg/mL
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using total cell extracts of NIH3T3 fibroblasts cells
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HDAC4(9759)
mouse ... Hdac4(208727)
rat ... Hdac4(363287)
General description
Monoclonal Anti-Histone Deacetylase 4 (HDAC4) (mouse IgG2a isotype) is derived from the HDAC4-144 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human HDAC4 with C-terminal added lysine, conjugated to KLH. HDAC4 belongs to the class II of Mammalian HDACs.
Application
Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
- immunoprecipitation
Biochem/physiol Actions
Histone deacetylase (HDAC) catalyzes the deacetylation of histones. HDAC4 is implicated in transcriptional repression. It dynamically migrates between nucleus and cytoplasm through its nuclear import and export signals. Interaction of HDAC4 with 14-3-3 and myocyte enhancer factor-2 (Mef2) proteins disturbs such shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Erasers of Histone Acetylation: The Histone Deacetylase Enzymes
Seto E, et al.
Cold Spring Harbor Perspectives in Biology, 6 (2014)
Histone deacetylase 4 possesses intrinsic nuclear import and export signals
Wang AH, et al.
Molecular and Cellular Biology, 21, 5992-6005 (2001)
Toon Verheyen et al.
Nucleic acids research, 43(12), 5771-5784 (2015-05-21)
Protein phosphatase-1 (PP1) is a key regulator of transcription and is targeted to promoter regions via associated proteins. However, the chromatin binding sites of PP1 have never been studied in a systematic and genome-wide manner. Methylation-based DamID profiling in HeLa