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Merck

W4125

Williams′ Medium E

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Medium E, Williams E Medium, Williams’ E medium

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352207
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form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L
glucose: 2.0 g/L
phenol red: 0.0107 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

General description

Williams′ E medium is a rich medium developed by Williams′ and Gunn. It is recommended for supporting long-term adult liver epithelial cell culture.

Application

Williams′ E medium has been used in culturing mice/rat primary hepatocytes.

Preparation Note

Formulated to contain 10.8 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.


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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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M Herranz-Itúrbide et al.
Redox biology, 40, 101841-101841 (2021-01-26)
Liver is a unique organ in displaying a reparative and regenerative response after acute/chronic damage or partial hepatectomy, when all the cell types must proliferate to re-establish the liver mass. The NADPH oxidase NOX4 mediates Transforming Growth Factor-beta (TGF-β) actions
Etienne Guillocheau et al.
The Journal of nutritional biochemistry, 63, 19-26 (2018-10-14)
Trans-palmitoleic acid (trans-C16:1 n-7 or trans-Δ9-C16:1, TPA) is believed to improve several metabolic parameters according to epidemiological data. TPA may mainly come from direct intakes: however, data are inconsistent due to its very low amount in foods. Instead, TPA might
D M Toivola et al.
Journal of cell science, 110 ( Pt 1), 23-33 (1997-01-01)
The importance of protein phosphatases in the maintenance of cytoskeletal structure is supported by the serious liver injury caused by microcystin-LR, a hepatotoxic inhibitor of type-1 and type-2A serine/threonine protein phosphatases. We used the microcystin-LR-induced cell injury as a model



Global Trade Item Number

SKUGTIN
W4125-10X1L04061837537974