48-681MAG
MILLIPLEX® Multi-Pathway Signaling Panel
Premixed Multi-Pathway Total 9-Plex Cell Signaling Panel
Synonym(s):
Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay, Signal Transduction Panel, MAPK STAT TCR assay, multi-pathway signaling panel, multi-target phosphorylation panel, phosphoprotein multiplex assay, rat cell signaling panel, rat intracellular signaling biomarkers
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About This Item
NACRES:
NA.47
UNSPSC Code:
12161503
Product Name
MILLIPLEX® Multi-Pathway Signaling Panel, Premixed Multi-Pathway Total 9-Plex Cell Signaling Panel
Quality Level
species reactivity
human, mouse, rat
manufacturer/tradename
Milliplex®
packaging
1 ea of
technique(s)
multiplexing: suitable
input
cell lysate
detection method
fluorometric (Luminex® xMAP® technology)
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Preparation Note
Recommended storage for kit components is 2 - 8°C
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
MAPMATE is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Features and Benefits
- Simultaneously detect 9 total proteins across key signaling pathways
- Covers MAPK, STAT, Akt, NFκB, and CREB pathways for broad cellular profiling
- Magnetic bead-based format ensures reproducibility and efficient processing
- Compatible with cell lysates from diverse experimental conditions
- Ideal for signal transduction, oncology, immunology, and toxicology research
- Fast, convenient alternative to Western blotting and immunoprecipitation procedures
General description
This panel includes total proteins from multiple signaling pathways that regulate cell survival, proliferation, inflammation, and transcriptional activity. ERK1/2, JNK, and p38 are core components of the MAPK pathway involved in stress and growth responses. Akt regulates cell survival and metabolism. NFκB controls inflammatory and immune signaling. STAT3 and STAT5A/B mediate cytokine-driven transcriptional regulation. CREB is a transcription factor activated by cAMP signaling, and p70 S6 Kinase plays a role in protein synthesis and cell growth. Quantifying these total proteins supports pathway profiling and comparative analysis across experimental conditions.
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 9 analytes in human, mouse, or rat cell lysate samples.
Analytes Included: ERK/MAPK 1/2, Akt, STAT3, JNK, p70 S6 Kinase, NFκB, STAT5A/B, CREB, p38
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
Analytes Included: ERK/MAPK 1/2, Akt, STAT3, JNK, p70 S6 Kinase, NFκB, STAT5A/B, CREB, p38
Assay Characteristics: Refer to the assay protocol for details on cross-reactivity, buffer compatibility, lysate control, and recommended loading control (MAPmate™ Control Assays).
signalword
Danger
Storage Class
10 - Combustible liquids
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2
Certificates of Analysis (COA)
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Poonam Naik et al.
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Infections with multidrug-resistant Pseudomonas aeruginosa (MDR-PA) lead to poor clinical outcomes in endophthalmitis patients, and its interactions with the host immune system remain largely unknown. The current study aimed to determine the association of MDR-PA infection with the cytokine expression
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Aging is often accompanied by exacerbated activation of cell death-related signaling pathways and decreased energy metabolism. We hypothesized that transcranial near-infrared laser may increase intracellular signaling pathways beneficial to aging brains, such as those that regulate brain cell proliferation, apoptosis
James P McNamee et al.
International journal of radiation biology, 97(9), 1316-1323 (2021-05-29)
To assess the effects of 1800 MHz radiofrequency electromagnetic field (RF-EMF) exposure on the expression of signal transduction and antioxidant proteins in a human-derived A172 glioblastoma cell line. Adherent human-derived A172 glioblastoma cells (1.0 × 105 cells per 35 mm culture dish, containing 2 mL
Alyson L Essex et al.
Biology of sex differences, 13(1), 56-56 (2022-10-02)
Osteocytic microRNA21 (miR21) removal alters cytokine production and bone mass by modulating osteoclast and osteoblast differentiation and activity. Removing osteocytic miR21 increases osteoclast/osteoblast numbers and bone mass in male mice, whereas it decreases osteoclasts/osteoblasts without affecting bone mass in female
Tania Quesada-López et al.
Nature communications, 7, 13479-13479 (2016-11-18)
The thermogenic activity of brown adipose tissue (BAT) and browning of white adipose tissue are important components of energy expenditure. Here we show that GPR120, a receptor for polyunsaturated fatty acids, promotes brown fat activation. Using RNA-seq to analyse mouse
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 48-681MAG | 04055977168792 |
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