CC130
Human Vitronectin
recombinant, expressed in E. coli, liquid, 0.5 mg/mL, used as an effective xeno-free alternative to matrigel, suitable for human ES and iPS cell culture, PLURISTEM®
Synonym(s):
S-protein, Serum-spreading factor, V75
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
Escherichia coli, human
Form:
liquid
Technique(s):
cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable
Concentration:
0.5 mg/mL
Assay:
≥92% (SDS-PAGE)
Product Name
PluriSTEM-XF® Recombinant Vitronectin, Human pluripotent stem cell culture.
Quality Level
biological source
Escherichia coli, human
assay
≥92% (SDS-PAGE)
form
liquid
manufacturer/tradename
PluriSTEM®
concentration
0.5 mg/mL
technique(s)
cell attachment: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable
input
sample type: human embryonic stem cell(s)
UniProt accession no.
shipped in
dry ice
storage temp.
-10 to -25°C
General description
PluriSTEM Recombinant Vitronectin is an effective xeno-free alternative to matrigel and has been validated to successully culture human ES and iPS cells using PluriSTEM Human ES/iPS Medium (Millipore Cat. No. SCM130, SCM132), E8 and NutriStem human ES cell growth media.
PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.
PluriSTEM Recombinant Vitronectin is a truncated form of human vitronectin (gene 20-398 aa fragment) that was constructed with codon optimization, expressed in E. coli and purified as inclusing bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified. The final product is considered xeno-free.
Application
Research Category
Stem Cell Research
Cell Structure
ECM & Adhesion
Stem Cell Research
Cell Structure
ECM & Adhesion
Supports feeder-free and serum-free growth of human es/ips cells while maintaining xeno-free culture conditions.
Physical form
Product is supplied in a 20 mM pH 8.0 Tris-HCl buffer with a proprietary formulation of sucrose, EDTA, arginine, and DTT.
Preparation Note
Store at -20°C until expiration date on vial. Once thawed, product is stable at 2 - 8°C for two weeks. Thawed vitronectin can be aliquoted and stored at -20°C. Avoid additional freeze-thaw cycles. Product has been 0.2 μm filtered.
Analysis Note
Each lot is tested on human ES cells (H1) cultured using 5-10 μg recombinant vitronectin in 1 mL human ESC medium per well (6-well plate).
Legal Information
PLURISTEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mutsumi Kobayashi et al.
Stem cell reports, 17(3), 507-521 (2022-02-13)
In vitro expansion of human primordial germ cell-like cells (hPGCLCs), a pluripotent stem cell-derived PGC model, has proved challenging due to rapid loss of primordial germ cell (PGC)-like identity and limited cell survival/proliferation. Here, we describe long-term culture hPGCLCs (LTC-hPGCLCs), which
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CC130 | 04053252938511 |