MAB1637
Anti-β-Tubulin III Antibody
CHEMICON®, mouse monoclonal, TU-20
Synonym(s):
Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Product Name
Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1), ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
TU-20 (Similar to TUJ1), monoclonal
species reactivity
rat, mouse, avian, human, bovine, monkey, pig, sheep
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TUBB3(10381)
Analysis Note
Control
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.
Positive control: Brain tissue or neurons of any type.
Negative control: Non neural tissue or Astrocytes.
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
Application
Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB with more than 55 product citations.
Immunohistochemistry:
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.
Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.
ELISA:
A previous lot of this antibody was used on ELISA.
Optimal working dilution must be determined by the end user.
On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
Immunocytochemistry:
1:100 to 1:500 dilution from a previous lot was used on fixed cells.
Immunoblotting:
1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.
ELISA:
A previous lot of this antibody was used on ELISA.
Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Biochem/physiol Actions
Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
55 kDa
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.
Immunogen
A synthetic peptide corresponding to amino acids 443-450 (ESEAQGPK) of human class III beta-tubulin conjugated to KLH.
Epitope: C-terminus
Other Notes
Replaces: 04-1049
Physical form
Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Function of mouse embryonic stem cell-derived supporting cells in neural progenitor cell maturation and long term expansion.
Guan, Y; Du, QA; Zhu, W; Zou, C; Wu, D; Chen, L; Zhang, YA
Testing null
Shelley R Hough et al.
PloS one, 4(11), e7708-e7708 (2009-11-06)
Commitment in embryonic stem cells is often depicted as a binary choice between alternate cell states, pluripotency and specification to a particular germ layer or extraembryonic lineage. However, close examination of human ES cell cultures has revealed significant heterogeneity in
Andrew Campbell et al.
Glia, 64(2), 227-239 (2015-10-16)
Astroglial dysfunction plays an important role in neurodegenerative diseases otherwise attributed to neuronal loss of function. Here we focus on the role of astroglia in ataxia-telangiectasia (A-T), a disease caused by mutations in the ataxia-telangiectasia mutated (ATM) gene. A hallmark
Yoshitaka Uno et al.
Human gene therapy, 22(6), 711-719 (2010-12-21)
We originally reported the use of vitamin E (α-tocopherol) as an in vivo vector of short-interfering RNA (siRNA) to the liver. Here, we apply our strategy to the brain. By combining high-density lipoprotein (HDL) as a second carrier with α-tocopherol-conjugated
Agnese Po et al.
The EMBO journal, 29(15), 2646-2658 (2010-06-29)
Hedgehog (Hh) pathway has a pivotal function in development and tumorigenesis, processes sustained by stem cells (SCs). The transcription factor Nanog controls stemness acting as a key determinant of both embryonic SC self-renewal and differentiated somatic cells reprogramming to pluripotency
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1637 | 08436037122545 |
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