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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
M-58, monoclonal
Species reactivity:
human
Application:
IHC
Citations:
45
Product Name
Anti-Procollagen Type I Antibody, NT, clone M-58, culture supernatant, clone M-58, Chemicon®
biological source
rat
Quality Level
antibody form
culture supernatant
clone
M-58, monoclonal
species reactivity
human
should not react with
rat, hamster, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
isotype
IgG2α
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
human ... COL1A1(1277)
Application
Detect Procollagen Type I using this Anti-Procollagen Type I Antibody, N-terminus, clone M-58 validated for use in IH(P).
Immunohistochemistry: 1:10,000-1:50,000 (fresh frozen tissue sections.)
Suitable for formaldehyde/formalin fixed paraffin sections, suggested starting dilution 1:100-1:1000, after treatment with 1% trypsin, 20 minutes at room temperature. Does not stain mature collagen fibers in tissue. Localized intracellularly in cells producing pro-collagen I. Not recommended for Western Blots Optimal working dilutions must be determined by end user.
Suitable for formaldehyde/formalin fixed paraffin sections, suggested starting dilution 1:100-1:1000, after treatment with 1% trypsin, 20 minutes at room temperature. Does not stain mature collagen fibers in tissue. Localized intracellularly in cells producing pro-collagen I. Not recommended for Western Blots Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes Human pro-collagen I. MAB1912 labels the amino-terminal pro-peptide.
Analysis Note
Control
Skin tissue
Skin tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Kangkang Zhi et al.
Journal of experimental & clinical cancer research : CR, 29, 66-66 (2010-06-10)
The prognosis of gastric cancer patients is difficult to predict because of defects in establishing the surgical-pathological features. Cancer-associated fibroblasts (CAFs) have been found to play prominent role in promoting tumor growth, invasion and metastasis. Thus raises the hypothesis that
James W Reinhardt et al.
Frontiers in immunology, 12, 784401-784401 (2022-01-04)
Fibrocytes are hematopoietic-derived cells that directly contribute to tissue fibrosis by producing collagen following injury, during disease, and with aging. The lack of a fibrocyte-specific marker has led to the use of multiple strategies for identifying these cells in vivo.
John P McCook et al.
Clinical, cosmetic and investigational dermatology, 9, 167-174 (2016-08-16)
To examine the effect of sodium copper chlorophyllin complex on the expression of biomarkers of photoaged dermal extracellular matrix indicative of skin repair. Following a previously published 12-day clinical assessment model, skin biopsy samples from the forearms of four healthy
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1912 | 04053252465383 |