MAB4360
Anti-TRA-1-60 Antibody, clone TRA-1-60
clone TRA-1-60, Chemicon®, from mouse
Synonym(s):
TRA-1-60 Marker
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-TRA-1-60 Antibody, clone TRA-1-60, clone TRA-1-60, Chemicon®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
TRA-1-60, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
input
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
isotype
IgM
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PODXL(5420)
Related Categories
Analysis Note
Control
NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)
Application
Immunofluorescence: A previous lot was used in IF.
Western Blot: A previous lot of this antibody was used in WB.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: A previous lot of this antibody was used in IH.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Western Blot: A previous lot of this antibody was used in WB.
Immunoprecipitation: A previous lot of this antibody was used in IP.
Immunohistochemistry: A previous lot of this antibody was used in IH.
Flow Cytometry: A starting range of 10-20 µg/mL is suggested.
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.
Biochem/physiol Actions
This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
235/410 kDa
Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.
Immunogen
Human embryonal carcinoma cell line 2102Ep
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.
Preparation Note
Stable for 1 year at from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Product Selector Tool.
Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Sriram Bandi et al.
Journal of cell science, 125(Pt 5), 1274-1283 (2012-02-22)
Understanding the identity of lineage-specific cells arising during manipulations of stem cells is necessary for developing their potential applications. For instance, replacement of crucial functions in organ failure by transplantation of suitable stem-cell-derived cells will be applicable to numerous disorders
Tomoko Andoh-Noda et al.
Molecular brain, 8, 31-31 (2015-05-28)
Rett syndrome (RTT) is one of the most prevalent neurodevelopmental disorders in females, caused by de novo mutations in the X-linked methyl CpG-binding protein 2 gene, MECP2. Although abnormal regulation of neuronal genes due to mutant MeCP2 is thought to
Glycan stem-cell markers are specifically expressed by spermatogonia in the adult non-human primate testis.
Muller, T; Eildermann, K; Dhir, R; Schlatt, S; Behr, R
Human Reproduction null
Mahdieh Jadaliha et al.
PloS one, 7(6), e38532-e38532 (2012-06-23)
Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through
Livia Eiselleova et al.
The International journal of developmental biology, 52(4), 353-363 (2008-04-17)
Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. However, feeder cells differ in their capacity to support the growth of undifferentiated
Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB4360 | 04053252610073 |
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service