RSTREP-RO

Roche

Streptavidin, recombinant

Name: Streptavidin, recombinant
Brand: ROCHE

liquid, ≥17 units/mg protein, pH range 5.0-9.0

Synonym(s):

streptavidin

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.54

Product Name

Streptavidin, recombinant, liquid, ≥17 units/mg protein, pH range 5.0-9.0

biological source

Streptomyces sp. ( Streptomyces avidinii)

recombinant

expressed in E. coli

form

liquid

specific activity

≥17 units/mg protein

mol wt

~52 kDa

packaging

pkg of 1 mg (11721666001)
pkg of 5 mg (11721674001)

manufacturer/tradename

Roche

concentration

70-90%

technique(s)

protein array: suitable
protein purification: suitable

impurities

6-7% water

pH range

5.0-9.0

pI

7.4-7.7 (two main bands)

solubility

water: 10 mg/L (mA₄₀₅=100)

suitability

suitable for immunological tests

UniProt accession no.

P22629

shipped in

cooler

storage temp.

-10 to -25°C

Quality Level

100

Related Categories

Roche Life Science Products

Application

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

General description

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit binds 1 μg D-biotin at pH 8.9.

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Physical form

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A₂₈₂ = 3.1)
K_D: 10^-15M

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

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Production of Recombinant Streptavidin and Optimization of Refolding Conditions for Recovery of Biological Activity

Jouybari RM et al.

Reports of Biochemistry and Molecular Biology, 6(2), 178-185 (2018)

Automated combinatorial process for nanofabrication of structures using bioderivatized nanoparticles.

Dehlinger D, et al.

Journal of the Association for Laboratory Automation, 5, 267-276 (2007)

MINSTED fluorescence localization and nanoscopy.

Michael Weber et al.

Nature photonics, 15(5), 361-366 (2021-05-07)

We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point

Biophysical studies of the translation initiation pathway with immobilized mRNA analogs.

John E G McCarthy et al.

Methods in enzymology, 430, 247-264 (2007-10-05)

A growing number of biophysical techniques use immobilized reactants for the quantitative study of macromolecular reactions. Examples of such approaches include surface plasmon resonance, atomic force microscopy, total reflection fluorescence microscopy, and others. Some of these methods have already been

MINSTED nanoscopy enters the Ångström localization range.