17779
2,3,5-Triphenyl-tetrazolium chloride solution
suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC
Synonym(s):
TTC solution, TTC supplement, TTC solution, TTC supplement
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About This Item
Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
Molecular Weight:
334.80
UNSPSC Code:
41171614
NACRES:
NA.85
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3923356
InChI
1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1
SMILES string
[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4
InChI key
PKDBCJSWQUOKDO-UHFFFAOYSA-M
sterility
sterile (Filtered and Aseptic Handled)
form
liquid
shelf life
limited shelf life, expiry date on the label
mol wt
334.8 g/mol
application(s)
agriculture
environmental
food and beverages, microbiology
storage temp.
2-8°C
suitability
bacteria
Quality Level
Application
2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media
General description
2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.
Other Notes
(per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Keiichi Hishikari et al.
Cardiovascular research, 81(1), 123-132 (2008-09-23)
Increased expression of several subtypes of prostaglandin E(2) receptors (EP1-4) has recently been described in clinical and experimental myocardial ischaemia/reperfusion (I/R) injury. However, their pathophysiological significance in I/R remains obscure. Thus, we determined whether the activation of the prostanoid receptor
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Journal of neuroinflammation, 14(1), 151-151 (2017-07-30)
Cerebral ischemia is a leading cause of death and disability with limited treatment options. Although inflammatory and immune responses participate in ischemic brain injury, the molecular regulators of neuroinflammation after ischemia remain to be defined. Translocator protein 18 kDa (TSPO) mainly
Neel R Sodha et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 33(5), 906-913 (2008-03-04)
Ischemia-reperfusion (I/R) injury, often encountered clinically, results in myocardial apoptosis and necrosis. Hydrogen sulfide (H(2)S) is produced endogenously in response to ischemia and thought to be cardioprotective, although its mechanism of action is not fully known. This study investigates cardioprotection
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Antibiotics (Basel, Switzerland), 9(8) (2020-07-28)
This study investigated the effects of Brazilian Red Propolis (BRP) extract on seven-day-old multispecies subgingival biofilms. Mixed biofilm cultures containing 31 species associated with periodontal health or disease were grown for six days on a Calgary device. Then, mature biofilms
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