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Merck

B8522

Brilliant Blue G solution

Concentrate

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About This Item

CAS Number:
6104-58-1
NACRES:
NA.47
PubChem Substance ID:
24892075
UNSPSC Code:
12171500
MDL number:
MFCD00078482

Product Name

Brilliant Blue G solution, Concentrate

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI key

RWVGQQGBQSJDQV-UHFFFAOYSA-M

form

liquid

technique(s)

microbe id | staining: suitable

color

dark blue

suitability

suitable for (Suitable for for staining protein in polyacrylamide and agarose gels)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

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Analysis Note

This product has been tested for suitability on SDS-PAGE.

Application

Brilliant Blue G solution has been used for protein band detection after SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). It is also used for the protein staining of cells on microscopic slide.

General description

Brilliant Blue G is a methanol based stain which is especially designed for staining protein in polyacrylamide gels, but will also stain protein in agarose gels (IEF). The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Water is added directly to the bottle to produce 1 liter of ready-to-use staining solution.

Preparation Note

After dilution with deionized water to a final volume of one liter, solution will contain 0.1% (w/v) Brilliant Blue G, 25% (v/v) methanol and 5% (v/v) acetic acid.
One bottle equals one liter working solution. 300 ml of brilliant blue G solution concentrate is supplied in the bottle.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

target_organs

Eyes,Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 2

flash_point_f

49.5 °F - closed cup

flash_point_c

9.7 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000419649
0000419649
SLCN1989
SLCK9977
SLCF2517

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