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Merck

08124

Isoamylase from Pseudomonas sp.

ammonium sulfate suspension, ≥10,000,000 units/mg protein

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.68 (BRENDA, IUBMB)
MDL number:
MFCD00131418
Specific activity:
≥10,000,000 units/mg protein

Key Documents

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Product Name

Isoamylase from Pseudomonas sp., ammonium sulfate suspension, ≥10,000,000 units/mg protein

form

ammonium sulfate suspension

specific activity

≥10,000,000 units/mg protein

storage temp.

2-8°C

Quality Level

100

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Related Categories

Analysis Note

≥10 MU/mg protein

Application

Isoamylase from Pseudomonas sp. has been used in starch debranching and to measure the chain-length distributions.

Biochem/physiol Actions

Pseudomonas amyloderamosa is considered a popular source of isoamylase. In the presence of a surfactant, this isoamylase produces a series of maltooligosaccharides. Immobilized isoamylase makes it applicable with a polysaccharide matrix composed of agarose, cellulose, and raw corn starch.
Catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Other Notes

One unit causes an increase in A610 of 0.1 in 1 hour using a homogenized solution of rice starch as a substrate (pH 3.5, 40°C)

pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP2315
BCCN7550
BCCM9130
BCCL1638
BCCL5940

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Digestion properties of corn starch modified by $\alpha$-D-glucan branching enzyme and cyclodextrin glycosyltransferase
Li Y, et al.
Food Hydrocolloids, 89, 534-541 (2019)
Enzymatic conversions of starch
Tomasik P and Horton D
Advances in Carbohydrate Chemistry and Biochemistry, 68, 59-436 (2012)
Rupendra Mukerjea et al.
Carbohydrate research, 345(3), 449-451 (2009-12-26)
Amylose, a putative linear alpha-(1-->4)-glucan and a component of most starches, was isolated from potato, rice, and wheat starches by forming the 1-butanol complex in a solution of the starches. It previously had been found that these amyloses were incompletely
Lucio Gullo et al.
Pancreas, 38(4), 396-400 (2009-03-20)
To determine the type and frequency of pancreatic lesions detected by magnetic resonance cholangiopancreatography (MRCP) in subjects with asymptomatic pancreatic hyperenzymemia and to assess for a possible relationship between these lesions and the hyperenzymemia. From January 2005 to May 2008
Sebastian Streb et al.
The Plant cell, 20(12), 3448-3466 (2008-12-17)
Several studies have suggested that debranching enzymes (DBEs) are involved in the biosynthesis of amylopectin, the major constituent of starch granules. Our systematic analysis of all DBE mutants of Arabidopsis thaliana demonstrates that when any DBE activity remains, starch granules
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