A2278
N-Acetyl-D-glucosamine–Agarose
saline suspension
Product Name
N-Acetyl-D-glucosamine–Agarose, saline suspension
InChI
1S/C8H15NO6/c1-4(12)9-5(2-10)7(14)8(15)6(13)3-11/h2,5-8,11,13-15H,3H2,1H3,(H,9,12)/t5-,6+,7+,8+/m0/s1
SMILES string
CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO
InChI key
MBLBDJOUHNCFQT-LXGUWJNJSA-N
form
saline suspension
matrix
6% beaded agarose
matrix activation
divinyl sulfone
matrix attachment
hydroxyl
matrix spacer
5 atoms
capacity
≥25 mg/mL binding capacity (Triticum vulgaris lectin)
storage temp.
2-8°C
Quality Level
Application
N-Acetyl-D-glucosamine-Agarose has been used
- for the incubation of supernatant for the depletion of ficolin-3-deficient serum
- for detecting the lectin activity by ligand binding assays
- to produce ficolin 2 depleted serum
General description
N-Acetyl-D-glucosamine is used in affinity chromatography, protein chromatography, and carbohydrate matrices. N-Acetyl-D-glucosamine can be used to purify wheat germ (Triticum vulgaris) lectin.
Physical form
Suspension in 0.15 M NaCl, 0.01 M sodium phosphate, pH 6.8, containing 0.02% sodium azide
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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R Sullivan et al.
Biochemical and biophysical research communications, 162(1), 184-188 (1989-07-14)
Human ejaculated spermatozoa are heterogeneous and can be separated into two distinct populations according to their respective buoyant densities. In order to investigate the functional differences between these two types of spermatozoa, we have searched for the presence of galactosyltransferase.
M B Allen et al.
The Biochemical journal, 185(3), 577-582 (1980-03-01)
1. Under normal assay conditions the N-acetyl-D-glucosamine kinases from rat liver and kidney show a pH-dependent lag phase before reaching a steady state, which is probably due to reversible dissociation of the dimeric enzyme. 2. The enzyme catalyses the phosphorylation
J B Pitner et al.
Carbohydrate research, 324(1), 17-29 (2000-03-21)
The binding of Strep 9, a mouse monoclonal antibody (mAb) of the IgG3 subclass directed against the cell-wall polysaccharide of Group A Streptococcus (GAS), has been characterized. The intact antibody and proteolytic fragments of Strep 9 bind differently to GAS:
L Munthe-Fog et al.
Scandinavian journal of immunology, 65(4), 383-392 (2007-03-28)
Ficolin-2 (L-ficolin), derived from the FCN2 gene, is an innate immunity pattern recognition molecule found in human serum in which inter-individual variation in serum appears to be under genetic control. To validate and extend this finding, we developed a sandwich
M B Allen et al.
The Biochemical journal, 185(3), 565-575 (1980-03-01)
1. Procedures for the extensive purification in high yield of N-acetyl-D-glucosamine kinase from rat liver and kidney are described. The separation of this enzyme from hepatic glucokinase depended primarily on their differing behaviour on an affinity column of Sepharose--N-(6-aminohexanoyl)-2-amino-2-deoxy-D-glucopyranose. 2.
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