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Merck

A4929

N,N′-Bis(acryloyl)cystamine

BioReagent, suitable for electrophoresis

Synonym(s):

2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide

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About This Item

Empirical Formula (Hill Notation):
C10H16N2O2S2
CAS Number:
60984-57-8
Molecular Weight:
260.38
NACRES:
NA.25
PubChem Substance ID:
24890926
UNSPSC Code:
12352116
EC Number:
262-546-8
MDL number:
MFCD00036225

Product Name

N,N′-Bis(acryloyl)cystamine, BioReagent, suitable for electrophoresis

Quality Level

200

InChI key

DJVKJGIZQFBFGS-UHFFFAOYSA-N

InChI

1S/C10H16N2O2S2/c1-3-9(13)11-5-7-15-16-8-6-12-10(14)4-2/h3-4H,1-2,5-8H2,(H,11,13)(H,12,14)

SMILES string

C=CC(=O)NCCSSCCNC(=O)C=C

product line

BioReagent

form

powder

suitability

suitable for electrophoresis

storage temp.

−20°C

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Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.
BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000467137
0000467137
0000414165
0000414165
0000398898

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