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About This Item
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), WB
Citations:
11
biological source
goat
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
technique(s)
direct ELISA: 1:30,000, western blot: 1:30,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Related Categories
General description
Immunoglobulin A (IgA) is the major mammalian antibody isotype that is synthesized in the gut associated lymphoid tissues. This immunoglobulin isotype modulates intestinal microorganisms and has been implicated in the pathogenesis of IgA nephropathy. Anti-Mouse IgA (α-chain specific)-Alkaline Phosphatase antibody is specific for mouse IgA when tested against purified mouse IgA, IgG, and IgM myeloma proteins.
Immunogen
Purified mouse IgA
Application
E. coli lysates and purified pili were analyzed by western blot using alkaline phosphatase conjugated goat anti-mouse IgA antibody as a secondary.
Anti-Mouse IgA (α-chain specific)-Alkaline Phosphatase antibody is suitable for use in ELISA. The product can also be used for western blot (1:30,000).
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Finn Terje Hegge et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(29), 10798-10803 (2004-07-14)
Several major bacterial pathogens and related commensal species colonizing the human mucosa express phosphocholine (PC) at their cell surfaces. PC appears to impact host-microbe biology by serving as a ligand for both C-reactive protein and the receptor for platelet-activating factor.
Duncan B Sutherland et al.
Current opinion in immunology, 24(3), 261-268 (2012-04-17)
Immunoglobulin A (IgA) is the most abundantly produced antibody isotype in mammals. The primary function of IgA is to maintain homeostasis at mucosal surfaces. IgA is generated in specialized gut associated lymphoid tissues (GALT) by T cell-dependent and T cell-independent
Young Youl Hyun et al.
Cell transplantation, 21(11), 2425-2439 (2012-04-25)
T-cell dysregulation plays an important role in the pathogenesis of immunoglobulin A nephropathy (IgAN). Adipose-derived stem cells (ASCs) have been reported to be able to prevent tissue damage through immune-modulating effects. To evaluate the effects of ASCs in high IgA
J S Sandhu et al.
Transgenic research, 9(2), 127-135 (2000-08-22)
Respiratory syncytial virus (RSV) is one of the most important pathogens of infancy and early childhood. Here a fruit-based edible subunit vaccine against RSV was developed by expressing the RSV fusion (F) protein gene in transgenic tomato plants. The F-gene
Gunnveig Grodeland et al.
Journal of immunology (Baltimore, Md. : 1950), 191(6), 3221-3231 (2013-08-21)
New influenza A viruses with pandemic potential periodically emerge due to viral genomic reassortment. In the face of pandemic threats, production of conventional egg-based vaccines is time consuming and of limited capacity. We have developed in this study a novel
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