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Merck

A8412

Bovine Serum Albumin solution

7.5% in DPBS, sterile-filtered, BioXtra, suitable for cell culture

Synonym(s):

Albumin solution from bovine serum, BSA solution

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About This Item

CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
7.0-8.0% protein basis (UV)
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Impurities:
≤13.0 EU/mL Endotoxin level
Concentration:
7.5% in DPBS
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biological source

bovine

Quality Level

description

low endotoxin

sterility

sterile-filtered

product line

BioXtra

assay

7.0-8.0% protein basis (UV)

form

liquid

mol wt

66.4 kDa

purified by

cold ethanol and heatshock fractionation

origin

USA origin

concentration

7.5% in DPBS

technique(s)

cell culture | mammalian: suitable

impurities

≤13.0 EU/mL Endotoxin level

pH

6.5-7.5

foreign activity

Mycoplasma test (Negative), cell growth >50%

shipped in

ambient

storage temp.

2-8°C

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Application

Qualified for use in cell culture applications.

Biochem/physiol Actions

Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media (SFM). It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.


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Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Pinar Sahin et al.
Fertility and sterility, 102(5), 1482-1490 (2014-09-23)
To evaluate the expression of mammalian target of rapamycin (mTOR) pathway molecules in mouse spermatogenesis and as well as its role during proliferation and meiotic initiation of spermatogenic cells. Experimental animal study. University. C57Balb-C adult male mice. Expressions of mTOR
Amanda J Shapiro et al.
Veterinary microbiology, 177(1-2), 154-161 (2015-03-18)
The seroprevalence of Coxiella burnetii (C. burnetii) in cats in eastern Australia is unknown, and the risk of transmission from cats to humans is undetermined. This study aimed to determine the exposure of cats to C. burnetii in four distinct
Eric M Kernfeld et al.
Immunity, 48(6), 1258-1270 (2018-06-10)
Thymus development is critical to the adaptive immune system, yet a comprehensive transcriptional framework capturing thymus organogenesis at single-cell resolution is still needed. We applied single-cell RNA sequencing (RNA-seq) to capture 8 days of thymus development, perturbations of T cell receptor rearrangement