BPQ24
BioPORTER® QuikEase™ Protein Delivery Kit
for intracellular delivery
Synonym(s):
BioPORTER Kit, Protein Delivery Kit
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About This Item
NACRES:
NA.26
UNSPSC Code:
41116133
General description
Contains 24 individual tubes of BioPORTER reagent
Application
BioPORTER® QuikEase™ Protein Delivery Kit has been used to deliver active granzyme B tagged with FITC into mouse neuroblastoma cell lines. Protein concentrations used for delivery were 6μg/ml which when stained for FITC-GrB yielded a 90% uptake rate.
The BioPORTER® QuikEase™ Protein Delivery Kit is the latest innovation in macromolecule delivery technology. It is a unique lipid-based formulation that allows for the intracellular delivery of proteins, peptides, and bioactive molecules such as enzymes and antibodies, into a broad range of cell types. The BioPORTER QuikEase kits contain individual tubes of the BioPORTER Reagent in a convenient, ready-to-use, single reaction format. Each tube contains sufficient material of the lyophilized BioPORTER Reagent to perform 1 reaction in a 6-well plate (or 35 mm dish), 4 reactions in a 24-well plate (or 16 mm wells), and 10 reactions in a 96-well plate.
BioPORTER® QuikEase™ Protein Delivery Kit has been used:
- for the delivery of single-chain antibody variable fragment (scFv) into HeLa cells
- for the transduction of QBI-293 cells with human Tau protein
- for the delivery of human granzyme B (GrB) in human cervical cancer cells
- for the delivery of epidermal fatty acid-binding protein (E-FABP) into nerve growth factor-differentiated PC12 cells (NGFDPC12)
Legal Information
BioPorter is a registered trademark of Gene Therapy Systems, Inc.
QuikEase is a trademark of Gene Therapy Systems, Inc.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
E. coli expression of a soluble, active single-chain antibody variable fragment containing a nuclear localization signal
Xiong H, et al.
Protein Expression and Purification, 66(2), 172-180 (2009)
Cancer vaccination drives Nanog-dependent evolution of tumor cells towards an immune-resistant and stem-like phenotype
Noh KH, et al.
Cancer Research, canres-ca3758 (2012)
Seeding of normal Tau by pathological Tau conformers drives pathogenesis of Alzheimer-like tangles
Guo JL and Lee VMY
The Journal of Biological Chemistry, 286(17), 15317-15331 (2011)