ECM001
Human Fibronectin
recombinant, expressed in HEK 293 cells, powder, suitable for cell culture
Synonym(s):
Fibronectin, CIG, Cold-insoluble globulin, FN
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About This Item
Product Name
Fibronectin human, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
biological source
human
recombinant
expressed in HEK 293 cells
sterility
sterile
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
259.5 kDa (The protein migrates as a higher band on SDS-PAGE due to glycosylation.)
packaging
pkg of 200 μg
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg endotoxin, tested
solubility
water: soluble
NCBI accession no.
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Quality Level
Gene Information
human ... FN1(2335)
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Analysis Note
The biological activity of recombinant human fibronectin was tested in culture by measuring the ability of immobilized fibronectin to support adhesion of CHO cells.
Application
Epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, endothelial cells.
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Fibronectin human has been used for coating cell culture plates for culturing endothelial progenitor cells. It has also been used as a component of Dulbecco′s modified Eagle′s medium (DMEM) in the in vitro matrigel invasion assay of glioblastoma cells.
Biochem/physiol Actions
Fibronectin (FN) is essential for embryonic development and mediates cell adhesion and migration functions. It mediates apoptosis in nasopharyngeal carcinoma (NPC). The plasma FN regulates thrombosis. Fibronectin functions as a ligand for the integrin receptor family and contains collagen, glycoprotein and glycosaminoglycan binding sites. Mutation in the FNI gene leads to an imbalance in the soluble and insoluble forms of fibronectin leading to glomerular deposits in hereditary kidney disease, glomerulopathy. Missense mutations in the FN1 gene are implicated in the skeletal dysplasias disorder, Spondylometaphyseal dysplasias.
Features and Benefits
- Human-derived fibronectin
- Produced through recombinant expression in HEK 293 cells
- Low endotoxin levels
- Absence of bacterial contamination
General description
Fibronectin (FN) is a glycoprotein with 5-9% carbohydrates present as N- and O-linked oligosaccharides. It belongs to the ligand glycoprotein family. The FN gene is localized to human chromosome 2q35. FN exists as two major isoforms, plasma-based soluble and extracellular matrix (ECM)-based insoluble form. Fibronectin structure comprises repeats of FNI, FNII and FNIII repeats. It also has fibrin-binding sites and exists as a dimer. Alternative splicing of the FN1 gene leads to the generation of many isoforms. This fibronectin protein is manufactured in human cells using an all-human production system with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.
Physical form
Supplied as a powder, lyophilized from CAPS buffered saline.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The association between hyperhomocysteinemia and cerebral small vessel disease
Yang Y, et al.
International Journal of Clinical and Experimental Medicine, 12, 13877-13883 (2019)
Jinting Wang et al.
American journal of translational research, 9(10), 4502-4511 (2017-11-10)
Fibronectin 1 (FN1) is a member of the glycoprotein family located on chromosome 2q35. It has been reported that FN1 is upregulated in many tumors, and its expression is negatively related to the prognosis and survival of cancer patients. Through
Jeannette Huaman et al.
Cells, 9(7) (2020-07-08)
Fibronectin (FN1) is an extracellular matrix protein gaining increasing attention for its multifaceted roles in cancer progression. Using our recently established circulating tumor cell (CTC) lines, we had demonstrated increased FN1 expression and enhanced migration in CTC lines, in comparison
Anna Lemańska-Perek et al.
Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 28(11), 1561-1567 (2019-10-10)
Fibronectin (FN) is a widely distributed glycoprotein which is present in different bodily fluids, on the surface of cells and in the extracellular matrix (ECM). It plays roles in various processes, including cell adhesion, migration, growth, proliferation, and tissue repair.
Wing S To et al.
Fibrogenesis & tissue repair, 4, 21-21 (2011-09-20)
Fibronectin (FN) is a ubiquitous extracellular matrix (ECM) glycoprotein that plays vital roles during tissue repair. The plasma form of FN circulates in the blood, and upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function
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