H6648

Hanks′ Balanced Salt solution

Name: Hanks′ Balanced Salt solution
Brand: SIGMA

Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HBSS

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About This Item

NACRES:

NA.75

UNSPSC Code:

12352207

Product Name

Hanks′ Balanced Salt solution, Modified, with sodium bicarbonate, without phenol red, calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO₃: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

shipped in

ambient

Quality Level

400

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Application

Hanks′ Balanced Salt solution has been used:

to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.
to measure the stability of the magnetic scaffold material in bone tissue engineering.
in Fluorometric Measurements of intracellular calcium levels [Ca²⁺]
for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood
For assessing airway inflammation in mice

General description

The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
Formulation: click here.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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A conceptually new type of bio-hybrid scaffold for bone regeneration.

A Tampieri et al.

Nanotechnology, 22(1), 015104-015104 (2010-12-08)

Magnetic bio-hybrid porous scaffolds have been synthesized, nucleating nano-apatite in situ on self-assembling collagen, in the presence of magnetite nano-particles. The magnetic phase acted as a sort of cross-linking agent for the collagen, inducing a chemico-physical-mechanical stabilization of the material

A novel route in bone tissue engineering: magnetic biomimetic scaffolds.

N Bock et al.

Acta biomaterialia, 6(3), 786-796 (2009-10-01)

In recent years, interest in tissue engineering and its solutions has increased considerably. In particular, scaffolds have become fundamental tools in bone graft substitution and are used in combination with a variety of bio-agents. However, a long-standing problem in the

Polymorphonuclear leukocytes isolated from umbilical cord blood as a useful research tool to study adherence to cell monolayers.

Janhavi Sharma et al.

Journal of immunological methods, 351(1-2), 30-35 (2009-10-06)

One of the initial steps in the inflammatory process involves the adherence and transmigration of circulating polymorphonuclear leukocytes (PMN) across the endothelial cell monolayer. One of the main constituents of the neutrophil phagosome that contributes to bacterial killing is myeloperoxidase

Extracellular NAD+ is an agonist of the human P2Y11 purinergic receptor in human granulocytes.

Iliana Moreschi et al.

The Journal of biological chemistry, 281(42), 31419-31429 (2006-08-24)

Micromolar concentrations of extracellular beta-NAD+ (NAD(e)+) activate human granulocytes (superoxide and NO generation and chemotaxis) by triggering: (i) overproduction of cAMP, (ii) activation of protein kinase A, (iii) stimulation of ADP-ribosyl cyclase and overproduction of cyclic ADP-ribose (cADPR), a universal

Protein disulfide isomerase plasma levels in healthy humans reveal proteomic signatures involved in contrasting endothelial phenotypes.

Percíllia Victória Santos de Oliveira et al.

Redox biology, 22, 101142-101142 (2019-03-15)

Redox-related plasma proteins are candidate reporters of protein signatures associated with endothelial structure/function. Thiol-proteins from protein disulfide isomerase (PDI) family are unexplored in this context. Here, we investigate the occurrence and physiological significance of a circulating pool of PDI in

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