I7633
IMDM
With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM
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About This Item
Product Name
Iscove′s Modified Dulbecco′s Medium, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
components
phenol red: yes
glucose: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
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Application
Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
General description
Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Preparation Note
Formulated to contain 17.7 grams of powder per liter of medium.
Supplement with 3.024 g/L sodium bicarbonate.
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
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