P2621

Phosphomannose Isomerase from Escherichia coli

Name: Phosphomannose Isomerase from <I>Escherichia coli</I>
Brand: SIGMA

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein

Synonym(s):

D-Mannose-6-phosphate ketol-isomerase, Mannose Phosphate Isomerase, PMI

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

5.3.1.8 (BRENDA, IUBMB)

MDL number:

MFCD00131899

Product Name

Phosphomannose Isomerase from Escherichia coli, recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein

recombinant

expressed in E. coli

form

ammonium sulfate suspension

specific activity

≥50 units/mg protein

storage temp.

2-8°C

Quality Level

200

Application

PMI is used to study cell wall synthesis and energy production. PMI has been used to study how EDTA and metal ions, such as Zn++, Co++, Fe++, Mn++ and Cu++., can affect recovery and thermal stability. It may be used to study PMI′s effect on various alginate biosynthetic enzymes such as phosphomannomutase (PMM), GDP-mannose pyrophosphorylase (GMP), and GDP-mannose dehydrogenase (GMD).

Biochem/physiol Actions

Phosphomannose Isomerase (PMI) catalyses the interconversion of mannose 6-phosphate (Man-6-P) and fructose 6-phosphate (Fru-6-P), which provides a link between glucose metabolism and mannosylation.

Other Notes

One unit will convert 1.0 μmole of D-mannose 6-phosphate to D-fructose 6-phosphate per min at pH 7.6 at 25 °C, using a coupled enzyme system with phosphoglucose isomerase and glucose-6-phosphate dehydrogenase.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical form

Supplied as a suspension in 3.2 M ammonium sulfate

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Potent, selective, and orally available benzoisothiazolone phosphomannose isomerase inhibitors as probes for congenital disorder of glycosylation Ia.

Russell Dahl et al.

Journal of medicinal chemistry, 54(10), 3661-3668 (2011-05-05)

We report the discovery and validation of a series of benzoisothiazolones as potent inhibitors of phosphomannose isomerase (PMI), an enzyme that converts mannose-6-phosphate (Man-6-P) into fructose-6-phosphate (Fru-6-P) and, more importantly, competes with phosphomannomutase 2 (PMM2) for Man-6-P, diverting this substrate

Characterization of the GDP-D-mannose biosynthesis pathway in Coxiella burnetii: the initial steps for GDP-β-D-virenose biosynthesis.

Craig T Narasaki et al.

PloS one, 6(10), e25514-e25514 (2011-11-09)

Coxiella burnetii, the etiologic agent of human Q fever, is a gram-negative and naturally obligate intracellular bacterium. The O-specific polysaccharide chain (O-PS) of the lipopolysaccharide (LPS) of C. burnetii is considered a heteropolymer of the two unusual sugars β-D-virenose and

Synthesis and evaluation of malonate-based inhibitors of phosphosugar-metabolizing enzymes: class II fructose-1,6-bis-phosphate aldolases, type I phosphomannose isomerase, and phosphoglucose isomerase.

Stéphanie Desvergnes et al.

Bioorganic & medicinal chemistry, 20(4), 1511-1520 (2012-01-25)

In the design of inhibitors of phosphosugar metabolizing enzymes and receptors with therapeutic interest, malonate has been reported in a number of cases as a good and hydrolytically-stable surrogate of the phosphate group, since both functions are dianionic at physiological

Extension of the substrate utilization range of Ralstonia eutropha strain H16 by metabolic engineering to include mannose and glucose.

Shanna Sichwart et al.

Applied and environmental microbiology, 77(4), 1325-1334 (2010-12-21)

The gram-negative facultative chemolithoautotrophic bacterium Ralstonia eutropha strain H16 is known for its narrow carbohydrate utilization range, which limits its use for biotechnological production of polyhydroxyalkanoates and possibly other products from renewable resources. To broaden its substrate utilization range, which

Mapmi gene contributes to stress tolerance and virulence of the entomopathogenic fungus, Metarhizium acridum.

Yueqing Cao et al.

Journal of invertebrate pathology, 108(1), 7-12 (2011-06-21)

Phosphomannose isomerase (PMI) catalyzes the reversible interconversion of fructose 6-phosphate (Fru-6-P) and mannose 6-phosphate (Man-6-P), providing a link between glycolysis and the mannose metabolic pathway. In this study, we identified pmi gene (Mapmi) from the entomopathogenic fungus, Metarhizium acridum, and

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