P9120

PNGase F from Elizabethkingia meningoseptica

Name: PNGase F from <I>Elizabethkingia meningoseptica</I>
Brand: SIGMA

recombinant, expressed in E. coli, set of 100 units nanomolar unit

Synonym(s):

N-Glycanase^®, N-Glycosidase F, PNGase F from Chryseobacterium meningosepticum, PNGase F from Flavobacterium meningosepticum, Peptide N-glycosidase

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About This Item

CAS Number:

83534-39-8

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.5.1.52 (BRENDA, IUBMB)

MDL number:

MFCD18839214

Product Name

PNGase F from Elizabethkingia meningoseptica, recombinant, expressed in E. coli, set of 100 units nanomolar unit

recombinant

expressed in E. coli

conjugate

(N-linked)

specific activity

≥10 units/mg protein

mol wt

36 kDa

packaging

set of 100 units nanomolar unit

shipped in

wet ice

storage temp.

2-8°C

Quality Level

200

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Related Categories

Glycobiology Profiling Tools

Protein Biology

Proteins & Enzymes

Application

PNGase F from Elizabethkingia meningoseptica has been used in deglycosylation

of recombinant soybean agglutinin (rSBA) in Nicotiana benthamiana (NbrSBA) and Solanum tuberosum (StrSBA)
of frontal cortical lysate to verify the glycosylation profile of β-secretase (BACE proteins)
of cell lysate for evaluating the siRNA silencing of cellular prion protein (PrP^c) post transfection

PNGase F is a glycosylasparaginase used to deglycosylate proteins. It is widely used in structure-function studies of glycoproteins.

Used to deglycosylate protein.

Biochem/physiol Actions

Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

PNGase F cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain. It deaminates the asparagine to aspartic acid, but leaves the oligosaccharide intact. PNGase F will not remove oligosaccharides containing α(1-3)-linked core fucose, commonly found in plant glycoproteins. A tripeptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F.

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 micromole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Packaging

Each set includes enzyme, two formulations of 5× reaction buffer (for routine and mass spectrometry downstream analysis), detergent and denaturation solutions

Legal Information

N-Glycanase is a registered trademark of Agilent Technologies Inc

pictograms

GHS06,GHS08,GHS05,GHS09

signalword

Danger

hcodes

H302,H311,H315,H317,H318,H334,H361d,H410

pcodes

P273 - P280 - P301 + P312 - P302 + P352 + P312 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

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PrPc activation induces neurite outgrowth and differentiation in PC12 cells: role for caveolin-1 in the signal transduction pathway

Pantera B, et al.

Journal of Neurochemistry, 110(1), 194-207 (2009)

The three-dimensional structure of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum.

G E Norris et al.

Structure (London, England : 1993), 2(11), 1049-1059 (1994-11-15)

Peptide:N-glycosidase F (PNGase F) is an enzyme that catalyzes the complete removal of N-linked oligosaccharide chains from glycoproteins. Often called an endoglycosidase, it is more correctly termed an amidase or glycosylasparaginase as cleavage is at the asparagine-sugar amide linkage. The

Mitochondrial respiratory inhibition and oxidative stress elevate beta-secretase (BACE1) proteins and activity in vivo in the rat retina

Xiong K, et al.

Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 181(3), 435-446 (2007)

High-yield expression of recombinant soybean agglutinin in plants using transient and stable systems.

Reynald Tremblay et al.

Transgenic research, 20(2), 345-356 (2010-06-19)

Soybean agglutinin (SBA) is a specific N-acetylgalactosamine-binding plant lectin that can agglutinate a wide variety of cells. SBA has great potential for medical and biotechnology-focused applications, including screening and treatment of breast cancer, isolation of fetal cells from maternal blood

PNGase F catalyzes de-N-glycosylation in a domestic microwave.

Hui Zhou et al.

Analytical biochemistry, 427(1), 33-35 (2012-04-21)

Common de-N-glycosylation protocols usually require a lengthy incubation time. Although pressure cycling technology or scientific microwave reactors can accelerate this enzyme reaction, they may not be easily accessible. In this brief report, we employed an alternative strategy using a standard

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