R1383
RPMI-1640 Medium
With ʟ-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Roswell Park Memorial Institute 1640 medium
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About This Item
Product Name
RPMI-1640 Medium, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
components
L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
shipped in
ambient
storage temp.
2-8°C
General description
RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Application
RPMI-1640 Medium has been used as a cell culture medium to culture human MCF-7 breast cancer cells and human castrate-resistant prostate cancer (CRPC) cell lines for various cell culture experiments. It has also been used to culture human islets to assess the oxidative stress response in primary human islets.
Preparation Note
Formulated to contain 8.4 grams of powder per liter of medium.
Supplement with 2.0 g/L sodium bicarbonate.
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Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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