SRP3110
M-CSF human
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
CSF-1, MGI-IM, Macrophage Colony Stimulating Factor
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352202
Product Name
M-CSF human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤1.0 ng/mL ED50
mol wt
36.8 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... CSF1(1435)
Application
M-CSF human has been used for the induction of osteoclastogenesis in human peripheral blood mononuclear cells.
Biochem/physiol Actions
M-CSF (macrophage colony-stimulating factor) is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells. M-CSF has been shown to play important roles in modulating dermal thickness, and male and female fertility. M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. M-CSF also works as a pleiotropic growth factor and plays a crucial role in reproduction and organogenesis. Absence of it can result in reduced growth, neurological and reproductive problems, developmental defects in the mammary gland, bone and pancreas. M-CSF is also involved in the pathogenesis of cancer.
General description
M-CSF (macrophage colony-stimulating factor) is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The protein works through its receptor CSF1R (colony stimulating factor 1 receptor). The human is reactive in murine systems, but the murine molecule exhibits no activity on human cells. Recombinant human M-CSF is a 36.8kDa homodimeric protein containing two 159 amino acid polypeptide subunits.
Other Notes
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Physical form
Lyophilized from 10 mM Sodium Phosphate, pH 8.0 + 75 mM NaCl.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The macrophage colony-stimulating factor, CSF-1.
E R Stanley
Methods in enzymology, 116, 564-587 (1985-01-01)
The inhibitory effect of microRNA-146a expression on bone destruction in collagen-induced arthritis.
Nakasa T, et al.
Arthritis and Rheumatism, 63, 1582-1590 (2011)
M-CSF in a new biomarker panel with HE4 and CA 125 in the diagnostics of epithelial ovarian cancer patients.
Bedkowska GE, et al.
Journal of Ovarian Research, 8, 27-27 (2015)
Janis L Abkowitz et al.
Transplantation, 88(3), 323-329 (2009-08-12)
Lysosomal storage diseases are devastating illnesses, in large part because of their neurologic consequences. Because significant morbidity occurs prenatally, in utero (IU) therapy is an attractive therapeutic approach. We studied the feasibility and efficacy of IU injections of monocytic cells
Huaqiang Yang et al.
Antiviral research, 151, 63-70 (2018-01-18)
Porcine reproductive and respiratory syndrome virus (PRRSV) causes severe economic losses to current swine production worldwide. Highly pathogenic PRRSV (HP-PRRSV), originated from a genotype 2 PRRSV, is more virulent than classical PRRSV and further exacerbates the economic impact. HP-PRRSV has
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