Anti-TRPM7 Antibody, clone DA5C7

Transient receptor potential cation channel subfamily M member 7 (UniProt: Q923J1; also known as EC:2.7.11.1, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTrpC7, Transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK) is encoded by the Trpm7 (also known as Chak, Ltrpc7) gene (Gene ID: 58800) in murine species. It is a bifunctional protein that exhibits both ion channel and kinase activities, essential for various physiological functions in mice. Subcellular localization studies reveal that TRPM7 is a multi-pass membrane protein and is predominantly found at the plasma membrane and within specialized cellular compartments, where it interacts with cytoskeletal proteins, influencing cell morphology and motility. It forms a non-selective cation channel with notable permeability to divalent cations such as Mg²⁺ and Zn²⁺, facilitating their influx into cells, which is critical for processes like embryonic development and cellular signaling. It is ubiquitously expressed across multiple tissues, including brain, skeletal muscle and, with stronger signals in kidney, heart, liver and spleen. Its also expressed in immune cells, indicating its fundamental role in cellular homeostasis and signaling pathways. Dysregulation of TRPM7 has been implicated in various diseases, including neurodegenerative disorders, cancer, and cardiac dysfunction, highlighting its potential as a therapeutic target. Research studies have elucidated the mechanisms by which TRPM7 contributes to neuronal survival and development, as well as its involvement in muscle regeneration and pyroptosis, a form of programmed cell death linked to inflammatory responses. A recent study reported that TRPM7 is crucial for early embryos because it maintains magnesium (Mg2+) levels, protects against oxidative stress, and promotes the proper gene activity required for development and cell differentiation before the blastocyst stage. (Ref.: Gupta, N., et al. (2023). Cell Rep. 42(10); 113232).

His-tagged, recombinant fragment corresponding to 254 amino acids from mouse TRPM7.

Quality Control Testing

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TRPM7 in mouse brain tissue lysate.





Western Blotting Analysis: A representative lot detected TRPM7 in Western Blotting application (Gupta, N., et al. (2023). Cell Rep. 42(10):113232).

Immunofluorescence Analysis: A representative lot detected TRPM7 in Immunofluorescence application (Gupta, N., et al. (2023). Cell Rep. 42(10):113232).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone DA5C7 is a mouse monoclonal antibody that specifically detects TRPM7. It targets an epitope within 254 amino acids from the C-terminal cytoplasmic domain.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.