Chymotrypsin Sequencing Grade

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl₂, pH 7.8).

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

For life science research only. Not for use in diagnostic procedures.