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47140

4-Fluoro-7-nitrobenzofurazan

suitable for fluorescence, BioReagent, ≥98.0% (HPLC)

Synonym(s):

NBD-F

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About This Item

Empirical Formula (Hill Notation):
C6H2FN3O3
CAS Number:
29270-56-2
Molecular Weight:
183.10
UNSPSC Code:
12352100
NACRES:
NA.32
PubChem Substance ID:
329757296
MDL number:
MFCD00010196
Beilstein/REAXYS Number:
1077571
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product line

BioReagent

Quality Level

200

assay

≥98.0% (HPLC)

mp

52-54 °C (lit.)

fluorescence

λex 470 nm; λem 528 nm in ethanol, λex 470 nm; λem 550 nm (Amino acid adducts)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-][N+](=O)c1ccc(F)c2nonc12

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

InChI key

PGZIDERTDJHJFY-UHFFFAOYSA-N

Application

4-Fluoro-7-nitrobenzofurazan (NBD-F) is used as a protein, amino acid and drug fluorescent labeling reagent in HPLC pre-column derivatization procedures.
Useful for fluorescent labeling of amino acids and amines in HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

235.4 °F - closed cup

flash_point_c

113 °C - closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF9196

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Yanting Song et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)
A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm
Hongyi Zhang et al.
Analytical and bioanalytical chemistry, 386(5), 1387-1394 (2006-09-06)
An in-capillary derivatization of amino acids and peptides with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was developed for their subsequent capillary electrophoretic analysis with laser-induced fluorescence detection (lambda (ex)=488 nm). The in-capillary derivatization was achieved in zone-passing mode by introducing successive plugs of sample
Hai Han et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(29), 3196-3202 (2011-03-05)
For a metabolomics study focusing on the analysis of aspartic and glutamic acid enantiomers, a fully automated two-dimensional HPLC system employing a microbore-ODS column and a narrowbore-enantioselective column was developed. By using this system, a detailed distribution of D-Asp and
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