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About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
eCl@ss:
32160702
Clone:
RM120, monoclonal
Species reactivity:
human
Application:
ELISA, IHC, WB
Citations:
1
biological source
rabbit
Quality Segment
antibody form
purified antibody
antibody product type
secondary antibodies
clone
RM120, monoclonal
species reactivity
human
technique(s)
ELISA: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG
shipped in
wet ice
target post-translational modification
unmodified
General description
(~150kDa, non-reduced) and heavy chain (~50kDa, reduced). No cross reactivity with other isotype of human IgG, and
mouse, rat, or goat IgG.
mouse, rat, or goat IgG.
Immunogen
Peptide corresponding to the hinge region of Human IgG4.
Application
Immunohistochemistry Analysis (IHC): 1 -10 μg/mL from a representative lot detected human IgG4 in human lymphoid tissue.
ELISA Analysis: The specificity and reactivity of a representative lot was confirmed by ELISA analyses.
ELISA Analysis: The specificity and reactivity of a representative lot was confirmed by ELISA analyses.
Rabbit-Anti-Human IgG4 Antibody, clone RM120 is an antibody against Human IgG4 for use in Western Blotting, Immunohistochemistry, ELISA.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Secondary Antibodies Adsorbed for Dual Labeling
Biochem/physiol Actions
This antibody reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.
Physical form
Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Evaluated by Western Blotting in human, mouse, rat, and goat IgG.
Western Blotting Analysis (WB): 2 μg/mL of this antibody detected human IgG in human, mouse, rat, and goat IgG.
Western Blotting Analysis (WB): 2 μg/mL of this antibody detected human IgG in human, mouse, rat, and goat IgG.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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