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05-1416

Anti-CD45 Antibody, clone IBL-5/25

Name: Anti-CD45 Antibody, clone IBL-5/25
Brand: MM

clone IBL-5/25, from rat

Synonym(s):

CD45 associated protein, CD45-associated protein, Lymphocyte phosphatase-associated phosphoprotein, PTPRC-associated protein, protein tyrosine phosphatase, receptor type, C-associated protein, protein tyrosine phosphatase, receptor type, c

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

IBL-5/25, monoclonal

Application:

FACS, IHC

Citations:

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Properties

Quality Segment

100

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

IBL-5/25, monoclonal

species reactivity

mouse

technique(s)

flow cytometry: suitable, immunohistochemistry: suitable

isotype

IgG

NCBI accession no.

NP_002829.2

UniProt accession no.

Q14761

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ptprc(19264)

Description

General description

CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

Approx. 147 kDa

Immunogen

Mouse lymph node cells.

Application

Anti-CD45 Antibody, clone IBL-5/25 is an antibody against CD45 for use in FC, IH.

Flow Cytometry Analysis: A previous lot detected CD45, clone IBL-5/25 in mouse peripheral blood lymphocytes

Biochem/physiol Actions

Reactivity with other species has not been determined.

This antibody reacts with all isoforms of mouse CD45 which is expressed on all haematopoietic cells except erythrocytes.
FUSION PARTNER: SP2/0 myeloma cell line

Physical form

Format: Purified

Purified rat monoclonal IgG in phosphate buffered saline containing 10 mM sodium azide.

Preparation Note

Store at 2-8°C for 12 months from date of receipt.

Analysis Note

Control
Mouse colon tissue

Evaluated by Immunohistochemistry in mouse colon tissue.
Immunohistochemistry Analysis: 1:100 dilution of this antibody detected CD45 in mouse colon tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: CBL1326

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WGK 2

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Not applicable

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