Skip to Content
Merck

Key Documents

View All Documentation

1.09211

Azure II (C.I.52010/52015)

for microscopy

Synonym(s):

Azure II, 3,7 Bis(dimethylamino)phenothiazin-5-ium chloride and 3-(Dimethylamino)-7-(methylamino)-phenothiazin-5-ium chloride

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

Linear Formula:
C16H18N3S · C15H16N3S ·2 Cl
CAS Number:
37247-10-2
Molecular Weight:
625.68
MDL number:
MFCD00080716
UNSPSC Code:
41116130
NACRES:
NA.21
Colour Index Number:
52010/52015
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Segment

400

form

solid

IVD

for in vitro diagnostic use

pH

2.9 (20 °C, 10 g/L in H2O)

solubility

100 g/L

bulk density

610 kg/m3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[s+]1c2c(nc3c1cc(cc3)N(CC)CC)ccc(c2)N(CC)CC, [Cl-].[Cl-].CNc1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](\C)C.CN(C)c4ccc5N=C6C=C\C(C=C6Sc5c4)=[N+](\C)C

InChI

1S/C16H18N3S.C15H15N3S.2ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;;/h5-10H,1-4H3;4-9H,1-3H3;2*1H/q+1;;;/p-1

InChI key

YOWZJZJLXUQHGF-UHFFFAOYSA-M

General description

Azure II (C.I. 52010/ 52015), is a dry dye for the preparation of a color staining solution used for the staining of blood and bone marrow smears of human origin. The present dye Azure II (C.I.52010/52015) - for microscopy is a defined dye mixture of azure I and methylene blue (1+1), for use in hematological stainings by addition of eosin Y. The staining of the nuclei is due of the molecular interaction of the Eosin Y dye and a complex of Azur B with DNA. Both dyes assemble to an Eosin Y - Azur B-DNA complex and the intensity of the resulting stain depends on the content of Azur B and the ratio of Azur B : Eosin Y.
Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library